ELISA/assay

Human beta-arrestin 2, ARRB2 ELISA Kit

MBS9301195

48-Strip-Wells

435 USD

ELISA Kit

Human beta-arrestin 2, ARRB2 ELISA Kit

beta-arrestin 2, ARRB2

ARRB2; Beta-arrestin-2; beta-arrestin-2; arrestin 3; arrestin beta-2; arrestin, beta 2; Arrestin beta-2

ARRB2

ARRB2; ARRB2; ARB2; ARR2; BARR2; ARB2; ARR2

ARRB2_HUMAN

Human

No significant cross-reactivity or interference between Human ARRB2 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 31.2 pg/ml - 1000 pg/ml. Sensitivity: 5.0 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ARRB2 concentrations in Human serum, plasma and other body fluids. Using Purified Human ARRB2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ARRB2 and ARRB2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ARRB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ARRB2 concentrations in Human serum, plasma and other body fluids. Using Purified Human ARRB2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ARRB2 and ARRB2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ARRB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

47496567

CAG29306.1

P32121

46,106 Da

ALK1 Signaling Events Pathway (137968); Activated NOTCH1 Transmits Signal To The Nucleus Pathway (576270); Arf6 Signaling Events Pathway (138034); Atypical NF-kappaB Pathway (137946); CXCR4-mediated Signaling Events Pathway (137910); Calcium Regulation In The Cardiac Cell Pathway (198906); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Disease Pathway (530764); Dopaminergic Synapse Pathway (469199)

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]

ARRB2: a member of the arrestin/beta-arrestin protein family. These proteins participate in agonist-mediated desensitization of G-protein-coupled receptors. Acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Expressed at high levels in the CNS and peripheral blood leukocytes. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 17p13. Cellular Component: postsynaptic membrane; endocytic vesicle; basolateral plasma membrane; postsynaptic density; cytoplasm; plasma membrane; dendritic spine; coated pit; cytoplasmic vesicle; nucleus; cytosol. Molecular Function: angiotensin receptor binding; protein domain specific binding; protein kinase B binding; type 2A serotonin receptor binding; follicle stimulating hormone receptor binding; alpha-1B adrenergic receptor binding; alpha-1A adrenergic receptor binding; protein binding; enzyme binding; G-protein-coupled receptor binding; ubiquitin protein ligase binding; protein complex binding; mitogen-activated protein kinase binding; protein complex scaffold; type 1 angiotensin receptor binding; D1 dopamine receptor binding; receptor binding. Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; follicle-stimulating hormone signaling pathway; positive regulation of receptor internalization; protein ubiquitination; negative regulation of caspase activity; adult walking behavior; protein transport; transforming growth factor beta receptor signaling pathway; receptor internalization; negative regulation of interleukin-6 production; detection of temperature stimulus involved in sensory perception of pain; arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; platelet activation; Notch signaling pathway; negative regulation of interleukin-12 production; negative regulation of natural killer cell mediated cytotoxicity; positive regulation of synaptic transmission, dopaminergic; negative regulation of interleukin-1 beta production; negative regulation of tumor necrosis factor production; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; G-protein coupled receptor internalization; positive regulation of protein ubiquitination; inhibition of NF-kappaB transcription factor; negative regulation of toll-like receptor signaling pathway; negative regulation of protein ubiquitination; brain development; positive regulation of calcium ion transport; blood coagulation

48-Strip-Wells

435 USD

96-Strip-Wells

600