catalog number :
MBS930093
products type :
ELISA Kit
products full name :
Rat Neuropeptide S, NPS ELISA Kit
products short name :
neuropeptide S
products name syn :
Rat Neuropeptide S (NPS) ELISA kit; neuropeptide S
other names :
neuropeptide S; Neuropeptide S; neuropeptide S; neuropeptide S
other gene names :
Nps; Nps
uniprot entry name :
NPS_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat NPS. No significant cross-reactivity or interference between Rat NPS and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Assay Type: Sandwich
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NPS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NPS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NPS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NPS bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001258042.1
ncbi gb acc num :
NM_001271113.1
ncbi mol weight :
9,763 Da
ncbi pathways :
Class A/1 (Rhodopsin-like Receptors) Pathway (1010164); G Alpha (q) Signalling Events Pathway (1010197); G Alpha (s) Signalling Events Pathway (1010194); GPCR Downstream Signaling Pathway (1010193); GPCR Ligand Binding Pathway (1010163); Gastrin-CREB Signalling Pathway Via PKC And MAPK (1010213); Peptide Ligand-binding Receptors Pathway (1010165); Signal Transduction Pathway (1010015); Signaling By GPCR Pathway (1010162)
uniprot summary :
NPS: Modulates arousal and anxiety. May play an important anorexigenic role. Binds to its receptor NPSR1 with nanomolar affinity to increase intracellular calcium concentrations. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular region. Biological Process: neuropeptide signaling pathway; positive regulation of synaptic transmission, GABAergic; visual learning; positive regulation of action potential; positive regulation of synaptic transmission, glutamatergic
size4 :
10x96-Strip-Wells