Human Mitochondrial uncoupling protein 2, UCP2 ELISA Kit | MyBioSource MBS930004 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Mitochondrial uncoupling protein 2, UCP2 ELISA Kit

catalog :

MBS930004

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS930004

products type :

ELISA Kit

products full name :

Human Mitochondrial uncoupling protein 2, UCP2 ELISA Kit

products short name :

uncoupling protein 2 (mitochondrial, proton carrier)

products name syn :

Human Mitochondrial uncoupling protein 2 (UCP2) ELISA kit; BMIQ4; SLC25A8; UCPH; uncoupling protein 2; uncoupling protein 2 (mitochondrial; proton carrier)

other names :

mitochondrial uncoupling protein 2; Mitochondrial uncoupling protein 2; mitochondrial uncoupling protein 2; UCP 2; solute carrier family 25 member 8; uncoupling protein 2 (mitochondrial, proton carrier); Solute carrier family 25 member 8; UCPH

products gene name :

UCP2

other gene names :

UCP2; UCP2; UCPH; BMIQ4; SLC25A8; SLC25A8; UCP 2

uniprot entry name :

UCP2_HUMAN

reactivity :

Human

sequence length :

309

specificity :

This assay has high sensitivity and excellent specificity for detection of Human UCP2. No significant cross-reactivity or interference between Human UCP2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, Cell lysates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: 15.6 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 3: Transport

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for UCP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UCP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UCP2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UCP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

13259541

ncbi acc num :

NP_003346.2

ncbi gb acc num :

NM_003355.2

uniprot acc num :

P55851

ncbi mol weight :

33,229 Da

ncbi pathways :

Electron Transport Chain Pathway (198860); Energy Metabolism Pathway (198907); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Metabolism Pathway (477135); Mitochondrial Uncoupling Proteins Pathway (160963); Respiratory Electron Transport, ATP Synthesis By Chemiosmotic Coupling, And Heat Production By Uncoupling Proteins. Pathway (105921); The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway (477137); The Fatty Acid Cycling Model Pathway (160964); The Proton Buffering Model Pathway (160965)

ncbi summary :

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'. [provided by RefSeq, Jul 2008]

uniprot summary :

UCP2: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Belongs to the mitochondrial carrier family. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter; Mitochondrial. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: mitochondrial inner membrane; integral to membrane. Biological Process: cellular metabolic process; response to superoxide; proton transport; cellular response to insulin stimulus; regulation of mitochondrial membrane potential; transport; cellular response to amino acid starvation; response to hypoxia; female pregnancy; mitochondrial transport; aging; negative regulation of apoptosis. Disease: Body Mass Index Quantitative Trait Locus 4

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage