catalog number :
MBS929997
products type :
ELISA Kit
products full name :
Human EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 ELISA Kit
products short name :
egf-like module containing, mucin-like, hormone receptor-like 1
products name syn :
Human EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1) ELISA kit; TM7LN3; egf-like module containing; mucin-like; hormone receptor-like sequence 1; egf-like module containing; mucin-like; hormone receptor-like 1
other names :
EGF-like module-containing mucin-like hormone receptor-like 1 isoform 2; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; EGF-like module receptor 1; egf-like module containing, mucin-like, hormone receptor-like sequence 1; egf-like module containing, mucin-like, hormone receptor-like 1; EGF-like module receptor 1; EMR1 hormone receptor
products gene name :
EMR1
other gene names :
EMR1; EMR1; TM7LN3; TM7LN3
uniprot entry name :
EMR1_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human EMR1. No significant cross-reactivity or interference between human EMR1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, Cell lysates, tissue homogenates. Assay Type: Sandwich. Detection Range: 25 pg/ml -1600 pg/ml. Sensitivity: The minimum detectable dose of human EMR1 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EMR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EMR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EMR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EMR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001243181.1
ncbi gb acc num :
NM_001256252.1
ncbi mol weight :
97,683 Da
ncbi pathways :
Class B/2 (Secretin Family Receptors) Pathway (106378); GPCR Ligand Binding Pathway (161020); GPCRs, Class B Secretin-like Pathway (198781); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)
ncbi summary :
This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
uniprot summary :
EMR1: Could be involved in cell-cell interactions. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 2; Receptor, GPCR. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: integral to plasma membrane; external side of plasma membrane. Molecular Function: G-protein coupled receptor activity; calcium ion binding. Biological Process: G-protein coupled receptor protein signaling pathway; cell adhesion
size4 :
10x96-Strip-Wells