Human Tropomyosin alpha-1 chain, TPM1 ELISA Kit | MyBioSource MBS929956 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Tropomyosin alpha-1 chain, TPM1 ELISA Kit

catalog :

MBS929956

quantity :

96 Strip Wells

price :

760 USD

product information

catalog number :

MBS929956

products type :

ELISA Kit

products full name :

Human Tropomyosin alpha-1 chain, TPM1 ELISA Kit

products short name :

tropomyosin 1 (alpha)

products name syn :

Human Tropomyosin alpha-1 chain (TPM1) ELISA kit; C15orf13; CMD1Y; HTM-alpha; TMSA; alpha tropomyosin; cardiomyopathy; hypertrophic 3; sarcomeric tropomyosin kappa; tropomyosin 1 alpha chain; tropomyosin 1 (alpha)

other names :

tropomyosin alpha-1 chain isoform 5; Tropomyosin alpha-1 chain; tropomyosin alpha-1 chain; alpha-tropomyosin; sarcomeric tropomyosin kappa; cardiomyopathy, hypertrophic 3; tropomyosin 1 (alpha); Alpha-tropomyosin; Tropomyosin-1

products gene name :

TPM1

other gene names :

TPM1; TPM1; CMH3; TMSA; CMD1Y; LVNC9; C15orf13; HTM-alpha; C15orf13; TMSA

uniprot entry name :

TPM1_HUMAN

reactivity :

Human

sequence length :

284

specificity :

This assay has high sensitivity and excellent specificity for detection of Human TPM1. No significant cross-reactivity or interference between Human TPM1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates, cell lysates. Detection Range: 25 pg/ml-1600 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 6.25 pg/ml

other info2 :

Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPM1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

27597085

ncbi acc num :

NP_000357.3

ncbi gb acc num :

NM_000366.5

uniprot acc num :

P09493

ncbi mol weight :

32,709 Da

ncbi pathways :

Cardiac Muscle Contraction Pathway 93344!!Cardiac Muscle Contraction Pathway 93992!!Dilated Cardiomyopathy Pathway 121494!!Dilated Cardiomyopathy Pathway 121285!!Hypertrophic Cardiomyopathy (HCM) Pathway 114229!!Hypertrophic Cardiomyopathy (HCM) Pathway 106591!!Muscle Contraction Pathway 106261!!Smooth Muscle Contraction Pathway 161025!!Striated Muscle Contraction Pathway 106262!!Striated Muscle Contraction Pathway 198903

ncbi summary :

This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

size :

96 Strip Wells

price :

760 USD