catalog number :
MBS929914
products type :
ELISA Kit
products full name :
Dog Somatostatin, SST ELISA Kit
products short name :
[somatostatin]
products name syn :
[Dog Somatostatin (SST) ELISA kit; SMST; somatostatin-14; somatostatin-28; somatostatin]
other names :
[somatostatin; Somatostatin; somatostatin; prosomatostatin]
products gene name :
[SST]
other gene names :
[SST; SST]
uniprot entry name :
SMS_CANFA
specificity :
This assay has high sensitivity and excellent specificity for detection of dog SST. No significant cross-reactivity or interference between dog SST and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.9 pg/ml -250 pg/ml. Sensitivity: The minimum detectable dose of dog SST is typically less than 0.98 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SST is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SST bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001003307.1
ncbi gb acc num :
NM_001003307.1
ncbi mol weight :
12,736 Da
ncbi pathways :
Class A/1 (Rhodopsin-like Receptors) Pathway (580548); G Alpha (i) Signalling Events Pathway (580576); GPCR Downstream Signaling Pathway (580574); GPCR Ligand Binding Pathway (580547); Gastric Acid Secretion Pathway (154414); Gastric Acid Secretion Pathway (154383); Peptide Ligand-binding Receptors Pathway (580549); Signal Transduction Pathway (580414); Signaling By GPCR Pathway (580546)
uniprot summary :
Function: Somatostatin inhibits the release of somatotropin.
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells