Human Complement factor H-related protein 5, CFHR5 ELISA Kit | MyBioSource MBS929912 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Complement factor H-related protein 5, CFHR5 ELISA Kit

catalog :

MBS929912

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS929912

products type :

ELISA Kit

products full name :

Human Complement factor H-related protein 5, CFHR5 ELISA Kit

products short name :

complement factor H-related 5

products name syn :

Human Complement factor H-related protein 5 (CFHR5) ELISA kit; CFHL5; FHR-5; FHR5; FLJ10549; MGC133240; factor H-related protein 5; complement factor H-related 5

other names :

complement factor H-related protein 5; Complement factor H-related protein 5; complement factor H-related protein 5; complement factor H-related 5

products gene name :

CFHR5

other gene names :

CFHR5; CFHR5; FHR5; CFHL5; FHR-5; CFHR5D; CFHL5; FHR5; FHR-5

uniprot entry name :

FHR5_HUMAN

reactivity :

Human

sequence length :

569

specificity :

This assay has high sensitivity and excellent specificity for detection of Human CFHR5. No significant cross-reactivity or interference between Human CFHR5 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml-40 ng/ml. Sensitivity: 0.156 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CFHR5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CFHR5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CFHR5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CFHR5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

13540563

ncbi acc num :

NP_110414.1

ncbi gb acc num :

NM_030787.3

uniprot acc num :

Q9BXR6

ncbi mol weight :

64,419 Da

ncbi summary :

This gene is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties, a region within repeats 5-7 having heparin binding and C reactive protein binding properties, and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3, binds C3b in a dose-dependent manner, and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated, but not causally linked, with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS). [provided by RefSeq, Jan 2010]

uniprot summary :

CFHR5: Involved in complement regulation. Defects in CFHR5 have been found in patients with atypical hemolytic uremic syndrome and may contribute to the disease. Atypical hemolytic uremic syndrome is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q31.3. Cellular Component: extracellular region. Biological Process: complement activation, alternative pathway. Disease: Cfhr5 Deficiency

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage