Bovine Ubiquitin-like protein ISG15, ISG15 ELISA Kit | MyBioSource MBS929641 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Bovine Ubiquitin-like protein ISG15, ISG15 ELISA Kit

catalog :

MBS929641

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS929641

products type :

ELISA Kit

products full name :

Bovine Ubiquitin-like protein ISG15, ISG15 ELISA Kit

products short name :

ISG15 ubiquitin-like modifier

products name syn :

Bovine Ubiquitin-like protein ISG15 (ISG15) ELISA kit; G1P2; IFI15; UCRP; interferon; alpha-inducible protein (clone IFI-15K) ; interferon-induced 17-kDa/15-kDa protein; interferon-stimulated protein; 15 kDa; ubiquitin cross-reactive protein; ISG15 ubiquitin-like modifier

other names :

ubiquitin-like protein ISG15; Ubiquitin-like protein ISG15; ubiquitin-like protein ISG15; boUCRP; ubiquitin cross-reactive protein; interferon-stimulated gene product 17; interferon-stimulated protein, 15 kDa; ISG15 ubiquitin-like modifier; Interferon-stimulated gene product 17; Ubiquitin cross-reactive protein; BoUCRP

products gene name :

ISG15

other gene names :

ISG15; ISG15; G1P2; ISG17; UCRP; BoUCRP

uniprot entry name :

ISG15_BOVIN

reactivity :

Bovine

sequence length :

154

specificity :

This assay has high sensitivity and excellent specificity for detection of bovine ISG15. No significant cross-reactivity or interference between bovine ISG15 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of bovine ISG15 is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ISG15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ISG15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ISG15 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ISG15 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

27805955

ncbi acc num :

NP_776791.1

ncbi gb acc num :

NM_174366.1

uniprot acc num :

O02741

ncbi mol weight :

17,310 Da

ncbi pathways :

Immune System Pathway (581534); Innate Immune System Pathway (581553); RIG-I-like Receptor Signaling Pathway (117267); RIG-I-like Receptor Signaling Pathway (116128); RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways (581593)

uniprot summary :

Function: Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Exhibits antiviral activity towards both DNA and RNA viruses. The secreted form of ISG15 can: induce natural killer cell proliferation, augment lymphokine-activated-killer (LAK) activity, induce dendritic cell maturation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.1 PublicationManual assertion based on experiment in:Ref.6

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage