Mouse Cathepsin G, CTSG ELISA Kit | MyBioSource MBS929501 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Cathepsin G, CTSG ELISA Kit

catalog :

MBS929501

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS929501

products type :

ELISA Kit

products full name :

Mouse Cathepsin G, CTSG ELISA Kit

products short name :

cathepsin G

products name syn :

Mouse Cathepsin G (CTSG) ELISA kit; CG; MGC23078; ; cathepsin G

other names :

cathepsin G preproprotein; Cathepsin G; cathepsin G; VSP; vimentin-specific protease; cathepsin G; Vimentin-specific protease; VSP

products gene name :

CTSG

other gene names :

Ctsg; Ctsg; VSP

uniprot entry name :

CATG_MOUSE

reactivity :

Mouse

sequence length :

261

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse CTSG. No significant cross-reactivity or interference between mouse CTSG and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.7 8 ng/ml -50 ng/ml. Sensitivity: The minimum detectable dose of mouse CTSG is typically less than 0.2 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CTSG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTSG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTSG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTSG bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

6681083

ncbi acc num :

NP_031826.1

ncbi gb acc num :

NM_007800.1

uniprot acc num :

P28293

ncbi mol weight :

29,096 Da

ncbi pathways :

Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Diabetes Pathways (574950); Disease Pathway (574923); Lysosome Pathway (99272); Lysosome Pathway (96865); Neuroactive Ligand-receptor Interaction Pathway (83250); Neuroactive Ligand-receptor Interaction Pathway (462); Regulation Of Insulin-like Growth Factor (IGF) Activity By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (574956); Renin-angiotensin System Pathway (83272)

ncbi summary :

This gene encodes a member of the peptidase S1 (chymotrypsin) family of serine endopeptidases. The encoded preproprotein is proteolytically processed to generate a mature protein product. This product appears to play a role in host defense, including inflammation and antigen processing. Homozygous knockout mice for this gene exhibit enhanced susceptibility to bacterial and fungal infection. [provided by RefSeq, Aug 2015]

uniprot summary :

CTSG: Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe- CH2Cl and phenylmethylsulfonyl fluoride. Belongs to the peptidase S1 family. Protein type: EC 3.4.21.20; Motility/polarity/chemotaxis; Protease. Cellular Component: extracellular matrix; extracellular space; membrane; intracellular membrane-bound organelle; cytoplasm; plasma membrane; intermediate filament; nucleus; secretory granule. Molecular Function: heparin binding; peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity. Biological Process: defense response to Gram-positive bacterium; positive regulation of immune response; response to lipopolysaccharide; protein processing; proteolysis; defense response to fungus

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage