Human 14-3-3 protein gamma, YWHAG ELISA Kit | MyBioSource MBS929301 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human 14-3-3 protein gamma, YWHAG ELISA Kit

catalog :

MBS929301

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS929301

products type :

ELISA Kit

products full name :

Human 14-3-3 protein gamma, YWHAG ELISA Kit

products short name :

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide

products name syn :

Human 14-3-3 protein gamma (YWHAG) ELISA kit; 14-3-3GAMMA; 14-3-3 gamma; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; gamma polypeptide

other names :

14-3-3 protein gamma; 14-3-3 protein gamma; 14-3-3 protein gamma; KCIP-1; 14-3-3 gamma; protein kinase C inhibitor protein 1; protein phosphatase 1, regulatory subunit 170; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma; Protein kinase C inhibitor protein 1; KCIP-1

products gene name :

YWHAG

other gene names :

YWHAG; YWHAG; PPP1R170; 14-3-3GAMMA; KCIP-1

uniprot entry name :

1433G_HUMAN

reactivity :

Human

sequence length :

247

specificity :

This assay has high sensitivity and excellent specificity for detection of human YWHAG. No significant cross-reactivity or interference between human YWHAG and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: 0.156 ng /ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for YWHAG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YWHAG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAG bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

21464101

ncbi acc num :

NP_036611.2

ncbi gb acc num :

NM_012479.3

uniprot acc num :

P61981

ncbi mol weight :

28,303 Da

ncbi pathways :

Calcium Regulation In The Cardiac Cell Pathway (198906); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Centrosome Maturation Pathway (105807); Class I PI3K Signaling Events Mediated By Akt Pathway (138020); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115)

ncbi summary :

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]

uniprot summary :

14-3-3 gamma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Homodimer. Interacts with SAMSN1. Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with PKA-phosphorylated AANAT. Highly expressed in brain, skeletal muscle, and heart. Belongs to the 14-3-3 family. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 7q11.23. Cellular Component: cytoplasmic vesicle membrane; focal adhesion; membrane; cytosol. Molecular Function: protein domain specific binding; protein kinase C inhibitor activity; insulin-like growth factor receptor binding; protein binding; protein kinase C binding; receptor tyrosine kinase binding. Biological Process: cellular response to insulin stimulus; regulation of neuron differentiation; apoptosis; organelle organization and biogenesis; regulation of signal transduction; negative regulation of protein kinase activity; regulation of synaptic plasticity; mitotic cell cycle; G2/M transition of mitotic cell cycle; protein targeting

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage