product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Lysosomal Pro-X carboxypeptidase, PRCP ELISA Kit
catalog :
MBS929190
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS929190
products type :
ELISA Kit
products full name :
Mouse Lysosomal Pro-X carboxypeptidase, PRCP ELISA Kit
products short name :
prolylcarboxypeptidase (angiotensinase C)
products name syn :
Mouse Lysosomal Pro-X carboxypeptidase (PRCP) ELISA kit; HUMPCP; MGC2202; PCP; angiotensinase C; lysosomal Pro-x carboxypeptidase; lysosomal carboxypeptidase C; proline carboxypeptidase; prolylcarboxypeptidase; prolylcarboxypeptidase isoform 1 preproprotein; prolylcarboxypeptidase (angiotensinase C)
other names :
lysosomal Pro-X carboxypeptidase; Lysosomal Pro-X carboxypeptidase; lysosomal Pro-X carboxypeptidase; proline carboxypeptidase; prolylcarboxypeptidase (angiotensinase C); Proline carboxypeptidase; Prolylcarboxypeptidase; PRCP
products gene name :
PRCP
other gene names :
Prcp; Prcp; PCP; HUMPCP; AI451719; 2510048K03Rik; 2610104A14Rik; PRCP
uniprot entry name :
PCP_MOUSE
reactivity :
Mouse
sequence length :
491
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse PRCP. No significant cross-reactivity or interference between mouse PRCP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates and Cell lysates. Assay Type: Sandwich. Detection Range: 37.5 pg/ml -2400 pg/ml. Sensitivity: The minimum detectable dose of mouse PRCP is typically less than 9.37 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRCP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRCP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRCP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRCP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
33469015
ncbi acc num :
NP_082519.1
ncbi gb acc num :
NM_028243.3
uniprot acc num :
Q7TMR0
ncbi mol weight :
55,027 Da
ncbi pathways :
Formation Of Fibrin Clot (Clotting Cascade) Pathway (575502); Hemostasis Pathway (575479); Intrinsic Pathway (575504); Protein Digestion And Absorption Pathway (172853); Protein Digestion And Absorption Pathway (171868)
uniprot summary :
PRCP: Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH. Belongs to the peptidase S28 family. Protein type: Protease; EC 3.4.16.2. Cellular Component: lysosome. Molecular Function: peptidase activity; carboxypeptidase activity; hydrolase activity; serine-type peptidase activity. Biological Process: plasma kallikrein-kinin cascade; negative regulation of systemic arterial blood pressure; proteolysis; angiogenesis involved in wound healing; regulation of blood vessel endothelial cell migration; glucose homeostasis
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

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