catalog number :
MBS928967
products type :
ELISA Kit
products full name :
Rat Regenerating islet-derived protein 3-alpha, REG3A ELISA Kit
products short name :
regenerating islet-derived 3 alpha
products name syn :
Rat Regenerating islet-derived protein 3-alpha (REG3A) ELISA kit; HIP; PAP; PAP-H; PAP1; PBCGF; REG-III; REG3; PAP homologous protein; hepatocarcinoma-intestine-pancreas; pancreatic beta cell growth factor; pancreatitis-associated protein; proliferation-inducing prote; regenerating islet-derived 3 alpha
other names :
regenerating islet-derived protein 3-alpha; Regenerating islet-derived protein 3-alpha; regenerating islet-derived protein 3-alpha; REG 3; regIII; REG-3-alpha; reg III-alpha; lithostathine 3; pancreatitis-associated protein 2; islet of Langerhans regenerating protein 3; regenerating islet-derived protein 3 alpha; regenerating islet-derived protein III-alpha; regenerating islet-derived 3 alpha; Islet of Langerhans regenerating protein 3; REG 3; Lithostathine 3; Pancreatitis-associated protein 2; RegIII; Regenerating islet-derived protein III-alpha; Reg III-alpha
products gene name :
REG3A
other gene names :
Reg3a; Reg3a; Pap2; PapII; Pap2; Reg3; REG-3-alpha; REG 3; Reg III-alpha
uniprot entry name :
REG3A_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat REG3A. No significant cross-reactivity or interference between rat REG3A and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: For the quantitative determination of rat regenerating islet-derived protein 3-alpha (REG3A) concentrations in serum, plasma, tissue homogenates. Detection Range: 6.25 ng/ml -400 ng/ml.
other info2 :
Sensitivity: The minimum detectable dose of rat REG3A is typically less than 1.56 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for REG3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any REG3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REG3A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REG3A bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001139318.1
ncbi gb acc num :
NM_001145846.2
ncbi mol weight :
19,599 Da
ncbi summary :
lectin-related secretory protein; overexpressed during the acute phase of pancreatitis [RGD, Feb 2006]
uniprot summary :
Function: Bactericidal C-type lectin. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway