Human Cytidine deaminase, CDA ELISA Kit | MyBioSource MBS928959 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Cytidine deaminase, CDA ELISA Kit

catalog :

MBS928959

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS928959

products type :

ELISA Kit

products full name :

Human Cytidine deaminase, CDA ELISA Kit

products short name :

cytidine deaminase

products name syn :

Human Cytidine deaminase (CDA) ELISA kit; CDD; cytidine aminohydrolase; cytosine nucleoside deaminase; small cytidine deaminase; cytidine deaminase

other names :

cytidine deaminase; Cytidine deaminase; cytidine deaminase; cytidine aminohydrolase; small cytidine deaminase; cytosine nucleoside deaminase; cytidine deaminase; Cytidine aminohydrolase

products gene name :

CDA

other gene names :

CDA; CDA; CDD; CDD

uniprot entry name :

CDD_HUMAN

reactivity :

Human

sequence length :

146

specificity :

This assay has high sensitivity and excellent specificity for detection of Human CDA. No significant cross-reactivity or interference between Human CDA and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CDA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CDA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CDA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CDA bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

11386157

ncbi acc num :

NP_001776.1

ncbi gb acc num :

NM_001785.2

uniprot acc num :

P32320

ncbi mol weight :

16,185 Da

ncbi pathways :

Drug Metabolism - Other Enzymes Pathway (83033); Drug Metabolism - Other Enzymes Pathway (428); Fluoropyrimidine Activity Pathway (198778); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Pyrimidine Metabolism Pathway (106281); Pyrimidine Metabolism Pathway (82946); Pyrimidine Metabolism Pathway (309); Pyrimidine Salvage Reactions Pathway (106284); Salvage Pathways Of Pyrimidine Deoxyribonucleotides (142286)

ncbi summary :

This gene encodes an enzyme involved in pyrimidine salvaging. The encoded protein forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. It is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in this gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias. [provided by RefSeq, Jul 2008]

uniprot summary :

CDA: This enzyme scavenge exogenous and endogenous cytidine and 2 -deoxycytidine for UMP synthesis. Belongs to the cytidine and deoxycytidylate deaminase family. Protein type: Hydrolase; Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - pyrimidine; EC 3.5.4.5. Chromosomal Location of Human Ortholog: 1p36.2-p35. Cellular Component: extracellular region; cytosol. Molecular Function: protein homodimerization activity; zinc ion binding; nucleoside binding; cytidine deaminase activity. Biological Process: cell surface receptor linked signal transduction; pyrimidine base metabolic process; cytosine metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; cytidine deamination; negative regulation of cell growth; pyrimidine salvage; negative regulation of nucleotide metabolic process; protein homotetramerization

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage