catalog number :
MBS928945
products type :
ELISA Kit
products full name :
Rat Transcobalamin-2, TCN2 ELISA Kit
products short name :
transcobalamin II; macrocytic anemia
products name syn :
Rat Transcobalamin-2 (TCN2) ELISA kit; D22S676; D22S750; TC2; transcobalamin II; transcobalamin II; macrocytic anemia
other names :
transcobalamin-2; Transcobalamin-2; transcobalamin-2; TC-2; TCII; TC II; transcobalamin II; transcobalamin 2; Transcobalamin II; TC II; TCII
products gene name :
TCN2
other gene names :
Tcn2; Tcn2; Tc2; Tcn2p; TC-2; TC II; TCII
uniprot entry name :
TCO2_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat TCN2. No significant cross-reactivity or interference between Rat TCN2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm
other info2 :
Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 2: Anion transport. Protein Biological Process 3: Cobalt transport
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TCN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TCN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TCN2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TCN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_071979.1
ncbi gb acc num :
NM_022534.1
ncbi mol weight :
47,420 Da
ncbi pathways :
One Carbon Metabolism Pathway 219770!!Vitamin Digestion And Absorption Pathway 199553!!Vitamin Digestion And Absorption Pathway 199538
ncbi summary :
component of plasma cobalamin (Cbl; vitamin B12) transport to all cells [RGD, Feb 2006]
uniprot summary :
Function: Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells