Human Tomoregulin-2, TMEFF2 ELISA Kit | MyBioSource MBS928793 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Tomoregulin-2, TMEFF2 ELISA Kit

catalog :

MBS928793

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS928793

products type :

ELISA Kit

products full name :

Human Tomoregulin-2, TMEFF2 ELISA Kit

products short name :

transmembrane protein with EGF-like and two follistatin-like domains 2

products name syn :

Human Tomoregulin-2 (TMEFF2) ELISA kit; HPP1; TENB2; TPEF; TR; tomoregulin; transmembrane protein TENB2; transmembrane protein with EGF-like and two follistatin-like domains 2

other names :

tomoregulin-2; Tomoregulin-2; tomoregulin-2; TR-2; transmembrane protein TENB2; hyperplastic polyposis protein 1; cancer/testis antigen family 120, member 2; transmembrane protein with EGF-like and two follistatin-like domains 2; Hyperplastic polyposis protein 1; Transmembrane protein with EGF-like and two follistatin-like domains

products gene name :

TMEFF2

other gene names :

TMEFF2; TMEFF2; TR; HPP1; TPEF; TENB2; CT120.2; HPP1; TENB2; TPEF; UNQ178/PRO204; TR-2

uniprot entry name :

TEFF2_HUMAN

reactivity :

Human

sequence length :

374

specificity :

This assay has high sensitivity and excellent specificity for detection of human TMEFF2. No significant cross-reactivity or interference between human TMEFF2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 28 pg/ml -1800 pg/ml. Sensitivity: The minimum detectable dose of human TMEFF2 is typically less than 7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TMEFF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMEFF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEFF2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEFF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

12383051

ncbi acc num :

NP_057276.2

ncbi gb acc num :

NM_016192.2

uniprot acc num :

Q9UIK5

ncbi mol weight :

41,428 Da

ncbi pathways :

Validated Targets Of C-MYC Transcriptional Repression Pathway (169353)

ncbi summary :

This gene encodes a member of the tomoregulin family of transmembrane proteins. This protein has been shown to function as both an oncogene and a tumor suppressor depending on the cellular context and may regulate prostate cancer cell invasion. Multiple soluble forms of this protein have been identified that arise from both an alternative splice variant and ectodomain shedding. Additionally, this gene has been found to be hypermethylated in multiple cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]

uniprot summary :

TMEFF2: may be a survival factor for hippocampal and mesencephalic neurons. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation. A member of the Cancer-Testis Antigen (CTA) superfamily. CTAs may play roles in embryonal development and tumor transformation or aspects of tumor progression. CTAs were once thought to be silenced in most normal adult tissues, with limited expression in fetal, placental, testis, and ovarian cells. These proteins are now known to be aberrantly expressed in various cancers and many are capable of eliciting humoral and cellular immune responses. Highly expressed in adult and fetal brain, spinal cord and prostate. Expressed in all brain regions except the pituitary gland, with highest levels in amygdala and corpus callosum. Expressed in the pericryptal myofibroblasts and other stromal cells of normal colonic mucosa. Expressed in prostate carcinoma. Down-regulated in colorectal cancer. Present in Alzheimer disease plaques. Down-regulated in tumor cell lines in response to a high level of methylation in the 5 region. The CpG island methylation correlates with TMEFF2 silencing in tumor cell lines. Belongs to the tomoregulin family. Three isoforms of the human protein are produced by alternative splicing. Isoform three is expressed weakly in testis and at high levels in normal and cancerous prostate. Note: This description may include information from RefSeq and UniProtKB. Protein type: Cancer Testis Antigen (CTA); Membrane protein, integral; Cell surface. Chromosomal Location of Human Ortholog: 2q32.3. Cellular Component: integral to membrane; extracellular region

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage