Mouse Chordin-like protein 1, CHRDL1 ELISA Kit | MyBioSource MBS928784 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Chordin-like protein 1, CHRDL1 ELISA Kit

catalog :

MBS928784

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS928784

products type :

ELISA Kit

products full name :

Mouse Chordin-like protein 1, CHRDL1 ELISA Kit

products short name :

chordin-like 1

products name syn :

Mouse Chordin-like protein 1 (CHRDL1) ELISA kit; RP6-141H5.1; CHL; NRLN1; VOPT; dA141H5.1; neuralin 1; neurogenesin-1; ventroptin; chordin-like 1

other names :

chordin-like protein 1 isoform 1; Chordin-like protein 1; chordin-like protein 1; neuralin-1; neurogenesin-1; ventroptin-beta; ventroptin-alpha; chordin-like 1; Neuralin-1; Neurogenesin-1; Ventroptin

products gene name :

CHRDL1

other gene names :

Chrdl1; Chrdl1; CHL; CHL1; VOPT; Nrln1; Ng1; Nrln1

uniprot entry name :

CRDL1_MOUSE

reactivity :

Mouse

sequence length :

447

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse CHRDL1. No significant cross-reactivity or interference between mouse CHRDL1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of mouse CHRDL1 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHRDL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHRDL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRDL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRDL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

167466239

ncbi acc num :

NP_001107857.1

ncbi gb acc num :

NM_001114385.1

uniprot acc num :

Q920C1

ncbi mol weight :

50,732 Da

ncbi pathways :

Signal Transduction Pathway (575318); Signaling By BMP Pathway (575413)

uniprot summary :

CHRDL1: Antagonizes the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embryonic bone formation. May also play an important role in regulating retinal angiogenesis through modulation of BMP4 actions in endothelial cells. Plays a role during anterior segment eye development. Defects in CHRDL1 are the cause of megalocornea type 1, X-linked (MGC1). MGC1 is an eye disorder in which the corneal diameter is bilaterally enlarged (greater than 13 mm) without an increase in intraocular pressure. It may also be referred to as anterior megalophthalmos, since the entire anterior segment is larger than normal. Features of megalocornea in addition to a deep anterior chamber include astigmatic refractive errors, atrophy of the iris stroma, miosis secondary to decreased function of the dilator muscle, iridodonesis, and tremulousness, subluxation, or dislocation of the lens. Whereas most affected individuals exhibit normal ocular function, complications include cataract development and glaucoma following lenticular dislocation or subluxation. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular region. Biological Process: neuron differentiation; nervous system development; ossification; eye development; multicellular organismal development; compound eye development; cell fate determination; cell differentiation; negative regulation of BMP signaling pathway

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage