ELISA/assay

Human Krueppel-like factor 4, KLF4 ELISA Kit

MBS928766

48-Strip-Wells

510 USD

ELISA Kit

Human Krueppel-like factor 4, KLF4 ELISA Kit

Kruppel-like factor 4 (gut)

Human Krueppel-like factor 4 (KLF4) ELISA kit; EZF; GKLF; endothelial Kruppel-like zinc finger protein; Kruppel-like factor 4 (gut)

Krueppel-like factor 4; Krueppel-like factor 4; Krueppel-like factor 4; gut-enriched krueppel-like factor; epithelial zinc finger protein EZF; endothelial Kruppel-like zinc finger protein; Kruppel-like factor 4 (gut); Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor

KLF4

KLF4; KLF4; EZF; GKLF; EZF; GKLF

KLF4_HUMAN

Human

513

This assay has high sensitivity and excellent specificity for detection of Human KLF4. No significant cross-reactivity or interference between Human KLF4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml-1200 pg/ml. Sensitivity: 4.68 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transcription/Transcription regulation. Protein Biological Process 3: Transcription

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KLF4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLF4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLF4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLF4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

194248077

NP_004226.3

NM_004235.4

O43474

54,671 Da

Developmental Biology Pathway (477129); Diabetes Pathways (105902); Disease Pathway (530764); Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway (169352); Synthesis, Secretion, And Deacylation Of Ghrelin Pathway (119538); Transcriptional Regulation Of White Adipocyte Differentiation Pathway (205243)

KLF4: Transcription factor; can act both as activator and as repressor. Binds the 5 -CACCC-3 core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. Interacts with POU5F1/OCT4 and SOX2. Interacts with MUC1 (via the C-terminal domain). Belongs to the krueppel C2H2-type zinc-finger protein family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription factor; DNA-binding; C2H2-type zinc finger protein. Chromosomal Location of Human Ortholog: 9q31. Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin. Molecular Function: protein binding; zinc ion binding; double-stranded DNA binding; phosphoinositide 3-kinase regulator activity; transcription factor activity. Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; post-embryonic camera-type eye development; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; negative regulation of interleukin-8 biosynthetic process; negative regulation of muscle hyperplasia; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; positive regulation of protein metabolic process; positive regulation of hemoglobin biosynthetic process; regulation of phosphoinositide 3-kinase activity; negative regulation of cell proliferation; post-embryonic hemopoiesis; epidermis morphogenesis; regulation of cell differentiation; negative regulation of protein kinase B signaling cascade; response to retinoic acid; negative regulation of heterotypic cell-cell adhesion; stem cell maintenance; positive regulation of telomerase activity; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; epidermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; mesodermal cell fate determination; negative regulation of phosphoinositide 3-kinase cascade

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800