Human Ferritin light chain, FTL ELISA Kit | MyBioSource MBS928657 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Ferritin light chain, FTL ELISA Kit

catalog :

MBS928657

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS928657

products type :

ELISA Kit

products full name :

Human Ferritin light chain, FTL ELISA Kit

products short name :

ferritin, light polypeptide

products name syn :

Human Ferritin light chain (FTL) ELISA kit; MGC71996; ferritin L subunit; ferritin L-chain; ferritin light chain; ferritin light polypeptide-like 3; ferritin; light polypeptide

other names :

ferritin light chain; Ferritin light chain; ferritin light chain; ferritin L-chain; ferritin L subunit; ferritin light polypeptide-like 3; ferritin, light polypeptide

products gene name :

FTL

other gene names :

FTL; FTL; LFTD; NBIA3; Ferritin L subunit

uniprot entry name :

FRIL_HUMAN

reactivity :

Human

sequence length :

175

specificity :

This assay has high sensitivity and excellent specificity for detection of Human FTL. No significant cross-reactivity or interference between Human FTL and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml-40 ng/ml. Sensitivity: 0.156 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 3: Iron storage

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FTL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FTL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FTL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FTL bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

20149498

ncbi acc num :

NP_000137.2

ncbi gb acc num :

NM_000146.3

uniprot acc num :

P02792

ncbi mol weight :

20,020 Da

ncbi pathways :

Clathrin Derived Vesicle Budding Pathway (119545); Golgi Associated Vesicle Biogenesis Pathway (119546); Iron Uptake And Transport Pathway (187191); Membrane Trafficking Pathway (106160); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); Porphyrin And Chlorophyll Metabolism Pathway (83021); Porphyrin And Chlorophyll Metabolism Pathway (407); Transmembrane Transport Of Small Molecules Pathway (106572); Trans-Golgi Network Vesicle Budding Pathway (106165)

ncbi summary :

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]

uniprot summary :

FTL: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS). It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene. Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3); also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels. Belongs to the ferritin family. Protein type: Oxidoreductase. Chromosomal Location of Human Ortholog: 19q13.33. Cellular Component: ferritin complex; membrane; cytosol. Molecular Function: identical protein binding; protein binding; ferric iron binding; iron ion binding. Biological Process: receptor-mediated endocytosis; iron ion homeostasis; cellular iron ion homeostasis; post-Golgi vesicle-mediated transport; iron ion transport; transmembrane transport. Disease: Hyperferritinemia With Or Without Cataract; L-ferritin Deficiency; Neurodegeneration With Brain Iron Accumulation 3

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage