ELISA/assay

Human VGF nerve growth factor inducible, VGF ELISA Kit

MBS928603

48-Strip-Wells

510 USD

ELISA Kit

Human VGF nerve growth factor inducible, VGF ELISA Kit

VGF nerve growth factor inducible

Human VGF nerve growth factor inducible (VGF) ELISA kit; neuro-endocrine specific protein VGF; neurosecretory protein VGF; VGF nerve growth factor inducible

neurosecretory protein VGF; Neurosecretory protein VGF; neurosecretory protein VGF; neuro-endocrine specific protein VGF; VGF nerve growth factor inducible

VGF

VGF; VGF; NERP-1; NERP-2

VGF_HUMAN

Human

615

This assay has high sensitivity and excellent specificity for detection of Human VGF. No significant cross-reactivity or interference between Human VGF and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

17136078

NP_003369.2

NM_003378.3

O15240

67,258 Da

This gene is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The structural organization of this gene is similar to that of the rat gene, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene. The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. [provided by RefSeq, Jul 2008]

VGF: May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7q22.1. Cellular Component: extracellular space; transport vesicle; cytoplasmic vesicle. Molecular Function: neuropeptide hormone activity; growth factor activity. Biological Process: response to dietary excess; generation of precursor metabolites and energy; response to cAMP; ovarian follicle development; sexual reproduction; insulin secretion; defense response to bacterium; response to cold; glucose homeostasis; response to insulin stimulus

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800