Human Apelin receptor, APLNR ELISA Kit | MyBioSource MBS928514 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Apelin receptor, APLNR ELISA Kit

catalog :

MBS928514

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

product information

catalog number :

MBS928514

products type :

ELISA Kit

products full name :

Human Apelin receptor, APLNR ELISA Kit

products short name :

[apelin receptor]

products name syn :

[Human Apelin receptor (APLNR) ELISA kit; AGTRL1; APJ; APJR; FLJ90771; FLJ96609; HG11; MGC45246; APJ (apelin) receptor; APJ receptor; G protein-coupled receptor APJ; HG11 orphan receptor; angiotensin II receptor-like 1; angiotensin receptor-like; apelin receptor]

other names :

[apelin receptor; Apelin receptor; apelin receptor; APJ receptor; HG11 orphan receptor; APJ (apelin) receptor; angiotensin receptor-like 1; G protein-coupled receptor APJ; G-protein coupled receptor APJ; angiotensin II receptor-like 1; G-protein coupled receptor HG11; apelin receptor; Angiotensin receptor-like 1; G-protein coupled receptor APJ; G-protein coupled receptor HG11]

products gene name :

[APLNR]

other gene names :

[APLNR; APLNR; APJ; APJR; HG11; AGTRL1; AGTRL1; APJ]

uniprot entry name :

APJ_HUMAN

reactivity :

Human

sequence length :

380

specificity :

This assay has high sensitivity and excellent specificity for detection of human APLNR. No significant cross-reactivity or interference between human APLNR and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 23.5 pg/ml -1500 pg/ml. Sensitivity: 5.8 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for APLNR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APLNR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APLNR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APLNR bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4885057

ncbi acc num :

NP_005152.1

ncbi gb acc num :

NM_005161.4

uniprot acc num :

P35414

ncbi mol weight :

42,660 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

ncbi summary :

This gene encodes a member of the G protein-coupled receptor gene family. The encoded protein is related to the angiotensin receptor, but is actually an apelin receptor that inhibits adenylate cyclase activity and plays a counter-regulatory role against the pressure action of angiotensin II by exerting hypertensive effect. It functions in the cardiovascular and central nervous systems, in glucose metabolism, in embryonic and tumor angiogenesis and as a human immunodeficiency virus (HIV-1) coreceptor. Two transcript variants resulting from alternative splicing have been identified. [provided by RefSeq, Jul 2009]

uniprot summary :

AGTRL1: Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia. Belongs to the G-protein coupled receptor 1 family. Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR. Chromosomal Location of Human Ortholog: 11q12. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; regulation of body fluid levels; heart development; gastrulation

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145