product summary
Loading...
company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat Macrophage metalloelastase, MMP12 ELISA Kit
catalog :
MBS928504
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS928504
products type :
ELISA Kit
products full name :
Rat Macrophage metalloelastase, MMP12 ELISA Kit
products short name :
matrix metallopeptidase 12 (macrophage elastase)
products name syn :
Rat Macrophage metalloelastase (MMP12) ELISA kit; HME; MGC138506; MME; macrophage elastase; macrophage metalloelastase; matrix metalloproteinase 12; matrix metalloproteinase 12 (macrophage elastase) ; matrix metallopeptidase 12 (macrophage elastase)
other names :
macrophage metalloelastase; Macrophage metalloelastase; macrophage metalloelastase; MME; MMP-12; matrix metalloproteinase-12; macrophage metalloelastase MMP-12; Matrix metalloproteinase-12; MMP-12
products gene name :
MMP12
other gene names :
MMP12; MMP12; MME; MMP-12
uniprot entry name :
MMP12_RABIT
reactivity :
Rat
sequence length :
464
specificity :
This assay has high sensitivity and excellent specificity for detection of rat MMP12. No significant cross-reactivity or interference between rat MMP12 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of rat MMP12 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP12 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
130487232
ncbi acc num :
NP_001076240.1
ncbi gb acc num :
NM_001082771.1
uniprot acc num :
P79227
ncbi mol weight :
53,584 Da
uniprot summary :
Function: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3 . Catalytic activity: Hydrolysis of soluble and insoluble elastin. Specific cleavages are also produced at 14-Ala- -Leu-15 and 16-Tyr- -Leu-17 in the B chain of insulin. Cofactor: Binds 4 calcium ions per subunit
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!