ELISA/assay

Human Calbindin, CALB1 ELISA Kit

MBS928501

48-Strip-Wells

510 USD

ELISA Kit

Human Calbindin, CALB1 ELISA Kit

calbindin 1, 28kDa

Human Calbindin (CALB1) ELISA kit; CALB; RTVL-H protein; calbindin 1; calbindin 1; (28kD) ; calbindin 1; 28kDa

calbindin; Calbindin; calbindin; D-28K; calbindin D28; RTVL-H protein; calbindin 1, (28kD); vitamin D-dependent calcium-binding protein, avian-type; calbindin 1, 28kDa; Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein, avian-type

CALB1

CALB1; CALB1; CALB; CAB27

CALB1_HUMAN

Human

261

This assay has high sensitivity and excellent specificity for detection of Human CALB1. No significant cross-reactivity or interference between Human CALB1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for calbindin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any calbindin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for calbindin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of calbindin bound in the initial step. The color development is stopped and the intensity of the color is measured.

4826655

NP_004920.1

NM_004929.2

P05937

30,025 Da

Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213307); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213276)

Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains 4 active calcium-binding domains, and 2 modified domains that presumably have lost their calcium-binding capacity. The neurons in brains of patients with Huntington disease are calbindin-depleted. [provided by RefSeq, Jul 2008]

CALB1: Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3 ,5 -cyclic nucleotide phosphodiesterase. Interacts with RANBP9. Belongs to the calbindin family. Protein type: Calcium-binding. Chromosomal Location of Human Ortholog: 8q21.3. Cellular Component: cell soma; axon; dendrite; synapse; cytosol; nucleus. Molecular Function: vitamin D binding; protein binding; zinc ion binding; calcium ion binding. Biological Process: cytosolic calcium ion homeostasis; long-term memory; short-term memory; regulation of synaptic plasticity; locomotory behavior

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800