Human Neurogenic locus notch homolog protein 3, NOTCH3 ELISA Kit | MyBioSource MBS928424 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Neurogenic locus notch homolog protein 3, NOTCH3 ELISA Kit

catalog :

MBS928424

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS928424

products type :

ELISA Kit

products full name :

Human Neurogenic locus notch homolog protein 3, NOTCH3 ELISA Kit

products short name :

Notch homolog 3 (Drosophila)

products name syn :

Human Neurogenic locus notch homolog protein 3 (NOTCH3) ELISA kit; CADASIL; CASIL; Notch homolog 3; Notch homolog 3 (Drosophila)

other names :

neurogenic locus notch homolog protein 3; Neurogenic locus notch homolog protein 3; neurogenic locus notch homolog protein 3; Notch homolog 3; notch 3

products gene name :

NOTCH3

other gene names :

NOTCH3; NOTCH3; IMF2; CASIL; CADASIL; Notch 3

uniprot entry name :

NOTC3_HUMAN

reactivity :

Human

sequence length :

2321

specificity :

This assay has high sensitivity and excellent specificity for detection of human NOTCH3. No significant cross-reactivity or interference between human NOTCH3 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates and Cell lysates. Assay Type: Sandwich. Detection Range: 125 pg/ml-8000 pg/ml. Sensitivity: The minimum detectable dose of human NOTCH3 is typically less than 31.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NOTCH3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NOTCH3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NOTCH3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NOTCH3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

134244285

ncbi acc num :

NP_000426.2

ncbi gb acc num :

NM_000435.2

uniprot acc num :

Q9UM47

ncbi mol weight :

243,631 Da

ncbi pathways :

Delta-Notch Signaling Pathway (198879); Dorso-ventral Axis Formation Pathway (114227); Dorso-ventral Axis Formation Pathway (472); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Notch Signaling Pathway (83062); Notch Signaling Pathway (169345); Notch Signaling Pathway (473); Notch-HLH Transcription Pathway (105939); Pre-NOTCH Expression And Processing Pathway (576265)

ncbi summary :

This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). [provided by RefSeq, Jul 2008]

uniprot summary :

NOTCH3: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Heterodimer of a C-terminal fragment N(TM) and a N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH3. Interacts with PSMA1. Interacts with HIF1AN. Ubiquitously expressed in fetal and adult tissues. Belongs to the NOTCH family. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 19p13.2-p13.1. Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; cytoplasm; integral to membrane; extracellular region; plasma membrane; cytosol; receptor complex; actin cytoskeleton. Molecular Function: protein binding; enzyme binding; calcium ion binding. Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; negative regulation of neuron differentiation; regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; forebrain development; neuron fate commitment; Notch receptor processing; gene expression. Disease: Cerebral Arteriopathy, Autosomal Dominant, With Subcortical Infarcts And Leukoencephalopathy; Lateral Meningocele Syndrome; Myofibromatosis, Infantile, 2

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage