product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human L-lactate dehydrogenase B chain, LDHB ELISA Kit
catalog :
MBS928418
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS928418
products type :
ELISA Kit
products full name :
Human L-lactate dehydrogenase B chain, LDHB ELISA Kit
products short name :
lactate dehydrogenase B
products name syn :
Human L-lactate dehydrogenase B chain (LDHB) ELISA kit; LDH-H; TRG-5; L-lactate dehydrogenase B; OTTHUMP00000165229; lactate dehydrogenase B
other names :
L-lactate dehydrogenase B chain; L-lactate dehydrogenase B chain; L-lactate dehydrogenase B chain; LDH heart subunit; lactate dehydrogenase H chain; renal carcinoma antigen NY-REN-46; epididymis secretory protein Li 281; lactate dehydrogenase B; LDH heart subunit; LDH-H; Renal carcinoma antigen NY-REN-46
products gene name :
LDHB
other gene names :
LDHB; LDHB; LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281; LDH-B; LDH-H
uniprot entry name :
LDHB_HUMAN
reactivity :
Human
sequence length :
334
specificity :
This assay has high sensitivity and excellent specificity for detection of human LDHB. No significant cross-reactivity or interference between human LDHB and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, urine, cerebrospinal fluid (CSF), tissue homogenates. Assay Type: Sandwich. Detection Range: 23.5 mU/ml-1500 mU/ml. Sensitivity: The minimum detectable dose of human LDHB is typically less than 5.8 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LDHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LDHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LDHB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LDHB bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
291575128
ncbi acc num :
NP_001167568.1
ncbi gb acc num :
NM_001174097.1
uniprot acc num :
P07195
ncbi mol weight :
36,638 Da
ncbi pathways :
Cysteine And Methionine Metabolism Pathway (104488); Cysteine And Methionine Metabolism Pathway (103421); Glycolysis / Gluconeogenesis Pathway (82926); Glycolysis / Gluconeogenesis Pathway (287); Glycolysis And Gluconeogenesis Pathway (198814); Metabolism Pathway (477135); Propanoate Metabolism Pathway (83004); Propanoate Metabolism Pathway (387); Pyruvate Metabolism Pathway (106205); Pyruvate Metabolism Pathway (82998)
ncbi summary :
This gene encodes an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on the X chromosome and on chromosome. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2010]
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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