catalog number :
MBS928369
products type :
ELISA Kit
products full name :
Mouse Membrane primary amine oxidase, AOC3 ELISA Kit
products short name :
amine oxidase, copper containing 3 (vascular adhesion protein 1)
products name syn :
Mouse Membrane primary amine oxidase (AOC3) ELISA kit; HPAO; SSAO; VAP-1; VAP1; amine oxidase; copper containing 3; copper amine oxidase; semicarbazide-sensitive amine oxidase; vascular adhesion protein 1; amine oxidase; copper containing 3 (vascular adhesion protein 1)
other names :
membrane primary amine oxidase; Membrane primary amine oxidase; membrane primary amine oxidase; VAP-1; copper amine oxidase; vascular adhesion protein 1; semicarbazide-sensitive amine oxidase; amine oxidase, copper containing 3; Copper amine oxidase; Semicarbazide-sensitive amine oxidase; SSAO; Vascular adhesion protein 1; VAP-1
products gene name :
AOC3
other gene names :
Aoc3; Aoc3; SSAO; VAP1; Vap1; SSAO; VAP-1
uniprot entry name :
AOC3_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse AOC3. No significant cross-reactivity or interference between Mouse AOC3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.25 ng/ml-80 ng/ml. Sensitivity: 0.31 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cell Adhesion. Protein Biological Process 3: Cell adhesion
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AOC3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AOC3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AOC3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AOC3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_033805.1
ncbi gb acc num :
NM_009675.2
ncbi mol weight :
84,534 Da
ncbi pathways :
Glycine, Serine And Threonine Metabolism Pathway (83149); Glycine, Serine And Threonine Metabolism Pathway (313); Phenylalanine Metabolism Pathway (83160); Phenylalanine Metabolism Pathway (327); Tyrosine Metabolism Pathway (83159); Tyrosine Metabolism Pathway (325); Beta-Alanine Metabolism Pathway (83165); Beta-Alanine Metabolism Pathway (335); Beta-alanine Biosynthesis I Pathway (142812); Threonine Degradation III (to Methylglyoxal) Pathway (142992)
uniprot summary :
AOC3: Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin- independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis. Belongs to the copper/topaquinone oxidase family. Protein type: Amino Acid Metabolism - phenylalanine; Other Amino Acids Metabolism - beta-alanine; Amino Acid Metabolism - tyrosine; Cell adhesion; EC 1.4.3.21; Oxidoreductase; Membrane protein, integral; Amino Acid Metabolism - glycine, serine and threonine. Cellular Component: extracellular space; microvillus; cell surface; membrane; intracellular membrane-bound organelle; cytoplasm; plasma membrane; integral to membrane. Molecular Function: amine oxidase activity; protein homodimerization activity; copper ion binding; protein heterodimerization activity; metal ion binding; oxidoreductase activity; cation channel activity; calcium ion binding; quinone binding. Biological Process: response to antibiotic; eating behavior; positive regulation of acute inflammatory response; positive regulation of leukocyte migration; regulation of blood pressure; amine metabolic process; cell adhesion; cation transport; leukocyte migration during inflammatory response
size4 :
10x96-Strip-Wells