ELISA/assay

Human upper zone of growth plate and cartilage matrix associated, UCMA ELISA Kit

MBS928312

48-Strip-Wells

510 USD

ELISA Kit

Human upper zone of growth plate and cartilage matrix associated, UCMA ELISA Kit

upper zone of growth plate and cartilage matrix associated

Human upper zone of growth plate and cartilage matrix associated (UCMA) ELISA kit; C10orf49; unique cartilage matrix-associated protein; upper zone of growth plate and cartilage matrix associated

unique cartilage matrix-associated protein; Unique cartilage matrix-associated protein; unique cartilage matrix-associated protein; Gla-rich protein; upper zone of growth plate and cartilage matrix associated; Gla-rich protein; GRP

UCMA

UCMA; UCMA; GRP; C10orf49; GRP/UCMA; C10orf49; Ucma-C; GRP

UCMA_HUMAN

Human

138

This assay has high sensitivity and excellent specificity for detection of Human UCMA. No significant cross-reactivity or interference between Human UCMA and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for UCMA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UCMA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UCMA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UCMA bound in the initial step. The color development is stopped and the intensity of the color is measured.

50400088

NP_660357.2

NM_145314.1

Q8WVF2

16,563 Da

This gene encodes a chondrocyte-specific, highly charged protein that is abundantly expressed in the upper immature zone of fetal and juvenile epiphyseal cartilage. The encoded protein undergoes proteolytic processing to generate a mature protein that is secreted into the extracellular matrix. The glutamic acid residues in the encoded protein undergo gamma carboxylation in a vitamin K-dependent manner. Undercarboxylation of the encoded protein is associated with osteoarthritis in humans. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015]

UCMA: May be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells in peripheral zones of fetal cartilage and at the cartilage-bone interface. Belongs to the UCMA family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 10p13. Cellular Component: extracellular space; proteinaceous extracellular matrix; perinuclear region of cytoplasm. Biological Process: negative regulation of osteoblast differentiation

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800