ELISA/assay

Human Multidrug resistance-associated protein 1, ABCC1 ELISA Kit

MBS928288

48-Strip-Wells

510 USD

ELISA Kit

Human Multidrug resistance-associated protein 1, ABCC1 ELISA Kit

ATP-binding cassette, sub-family C (CFTR/MRP) , member 1

Human Multidrug resistance-associated protein 1 (ABCC1) ELISA kit; ABC29; ABCC; DKFZp686N04233; DKFZp781G125; GS-X; MRP; MRP1; ATP-binding cassette; sub-family C; member 1; LTC4 transporter; leukotriene C (4) transporter; multidrug resistance protein; multiple drug resi; ATP-binding cassette; sub-family C (CFTR/MRP); member 1

multidrug resistance-associated protein 1; Multidrug resistance-associated protein 1; multidrug resistance-associated protein 1; LTC4 transporter; leukotriene C(4) transporter; ATP-binding cassette sub-family C member 1; ATP-binding cassette, sub-family C (CFTR/MRP), member 1; ATP-binding cassette sub-family C member 1; Leukotriene C(4) transporter; LTC4 transporter

ABCC1

ABCC1; ABCC1; MRP1; LTC4 transporter

MRP1_BOVIN

Human

1530

This assay has high sensitivity and excellent specificity for detection of human ABCC1. No significant cross-reactivity or interference between human ABCC1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: The minimum detectable dose of human ABCC1 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ABCC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ABCC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCC1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

27806339

NP_776648.1

NM_174223.1

Q8HXQ5

171,666 Da

ABC Transporters Pathway (84274); ABC Transporters Pathway (436); Irinotecan Pathway (198567); Vitamin Digestion And Absorption Pathway (199555); Vitamin Digestion And Absorption Pathway (199538)

Function: Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. May protect milk against xenobiotics. Hydrolyzes ATP with low efficiency 1 PublicationManual assertion based on experiment in:Ref.1

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800