product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Endothelial cell-selective adhesion molecule, ESAM ELISA Kit
catalog :
MBS928214
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS928214
products type :
ELISA Kit
products full name :
Mouse Endothelial cell-selective adhesion molecule, ESAM ELISA Kit
products short name :
endothelial cell adhesion molecule
products name syn :
Mouse Endothelial cell-selective adhesion molecule (ESAM) ELISA kit; W117m; 2310008D05Rik; HUEL (C4orf1) -interacting protein; LP4791 protein; endothelial cell adhesion molecule
other names :
endothelial cell-selective adhesion molecule; Endothelial cell-selective adhesion molecule; endothelial cell-selective adhesion molecule; endothelial cell-specific adhesion molecule
products gene name :
ESAM
other gene names :
Esam; Esam; Esam1; W117m; 2310008D05Rik; Esam1
uniprot entry name :
ESAM_MOUSE
reactivity :
Mouse
sequence length :
394
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse ESAM. No significant cross-reactivity or interference between mouse ESAM and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of mouse ESAM is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ESAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ESAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ESAM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ESAM bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
22094995
ncbi acc num :
NP_081378.1
ncbi gb acc num :
NM_027102.3
uniprot acc num :
Q925F2
ncbi mol weight :
41,810 Da
ncbi pathways :
Cell Adhesion Molecules (CAMs) Pathway (83266); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (575507); Hemostasis Pathway (575479); Leukocyte Transendothelial Migration Pathway (83280); Leukocyte Transendothelial Migration Pathway (494)
uniprot summary :
ESAM: Can mediate aggregation most likely through a homophilic molecular interaction. Protein type: Membrane protein, integral. Cellular Component: adherens junction; tight junction; membrane; plasma membrane; integral to membrane; cell junction. Biological Process: cell-cell adhesion; homophilic cell adhesion; cell adhesion; calcium-independent cell-cell adhesion
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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