catalog number :
MBS928156
products type :
ELISA Kit
products full name :
Human Cathelicidin antimicrobial peptide, CAMP ELISA Kit
products short name :
[cathelicidin antimicrobial peptide]
products name syn :
[Human Cathelicidin antimicrobial peptide (CAMP) ELISA kit; CAP18; CRAMP; FALL-39; FALL39; HSD26; LL37; ; cathelicidin antimicrobial peptide]
other names :
[cathelicidin antimicrobial peptide preproprotein; Cathelicidin antimicrobial peptide; cathelicidin antimicrobial peptide; 18 kDa cationic antimicrobial protein; cathelicidin antimicrobial peptide; 18 kDa cationic antimicrobial protein; CAP-18; hCAP-18]
products gene name :
[CAMP]
other gene names :
[CAMP; CAMP; LL37; CAP18; CRAMP; HSD26; CAP-18; FALL39; FALL-39; CAP18; FALL39; HSD26; CAP-18; hCAP-18]
uniprot entry name :
CAMP_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human CAMP. No significant cross-reactivity or interference between human CAMP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, tissue homogenates and cell culture supernates. Assay Type: Sandwich. Detection Range: 0.45 ng/ml -30 ng/ml. Sensitivity: The minimum detectable dose of human CAMP is typically less than 0.11 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CAMP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CAMP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAMP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAMP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_004336.3
ncbi gb acc num :
NM_004345.4
ncbi mol weight :
19,301 Da
ncbi pathways :
Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352); Tuberculosis Pathway (213780); Tuberculosis Pathway (213743)
ncbi summary :
This gene encodes a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation. [provided by RefSeq, Sep 2014]
uniprot summary :
CAMP: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity. Belongs to the cathelicidin family. Protein type: Cell cycle regulation; Vesicle; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 3p21.3. Cellular Component: extracellular space; specific granule; cell projection; cell wall; cytosol. Molecular Function: protein binding. Biological Process: interaction with host; positive regulation of angiogenesis; defense response to Gram-positive bacterium; innate immune response in mucosa; killing by host of symbiont cells; defense response to bacterium; positive regulation of cell proliferation; antibacterial humoral response; positive regulation of protein amino acid phosphorylation; defense response to Gram-negative bacterium
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells