catalog number :
MBS928106
products type :
ELISA Kit
products full name :
Rat ATP-binding cassette sub-family G member 5, ABCG5 ELISA Kit
products short name :
ATP-binding cassette, sub-family G (WHITE) , member 5
products name syn :
Rat ATP-binding cassette sub-family G member 5 (ABCG5) ELISA kit; STSL; ATP-binding cassette sub-family G member 5; ATP-binding cassette; subfamily G; member 5; sterolin 1; ATP-binding cassette; sub-family G (WHITE); member 5
other names :
ATP-binding cassette sub-family G member 5; ATP-binding cassette sub-family G member 5; ATP-binding cassette sub-family G member 5; sterolin 1; sterolin-1; ATP-binding cassette sub-family G (WHITE) member 5 (sterolin 1); ATP-binding cassette, sub-family G (WHITE), member 5 (sterolin 1); ATP-binding cassette, subfamily G (WHITE), member 5; Sterolin-1
products gene name :
ABCG5
other gene names :
Abcg5; Abcg5
uniprot entry name :
ABCG5_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat ABCG5. No significant cross-reactivity or interference between rat ABCG5 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 25 pg/ml -1600 pg/ml. Sensitivity: The minimum detectable dose of rat ABCG5 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ABCG5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ABCG5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCG5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCG5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_446206.2
ncbi gb acc num :
NM_053754.2
ncbi mol weight :
73,372 Da
ncbi pathways :
ABC Transporters Pathway (83427); ABC Transporters Pathway (436); ABC-family Proteins Mediated Transport Pathway (574474); ABCA Transporters In Lipid Homeostasis Pathway (574475); Bile Secretion Pathway (193141); Bile Secretion Pathway (193095); Fat Digestion And Absorption Pathway (194376); Fat Digestion And Absorption Pathway (194324); Lipid Digestion, Mobilization, And Transport Pathway (573951); Metabolism Pathway (573935)
ncbi summary :
ATP-transporter family member that may regulate absorption of dietary cholesterol; human homolog is mutated in sitosterolemia, a disease of hyperabsorption of cholesterol and other sterols [RGD, Feb 2006]
uniprot summary :
Function: Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.
size4 :
10x96-Strip-Wells