ELISA/assay

Rat Estrogen receptor beta, ESR2 ELISA Kit

MBS928082

48-Strip-Wells

510 USD

ELISA Kit

Rat Estrogen receptor beta, ESR2 ELISA Kit

estrogen receptor 2 (ER beta)

Rat Estrogen receptor beta (ESR2) ELISA kit; ER-BETA; ESR-BETA; ESRB; ESTRB; Erb; NR3A2; estrogen receptor beta; estrogen receptor beta 4; estrogen receptor 2 (ER beta)

estrogen receptor beta; Estrogen receptor beta; estrogen receptor beta; estrogen receptor 2 beta; nuclear receptor subfamily 3 group A member 2; estrogen receptor 2 (ER beta); Nuclear receptor subfamily 3 group A member 2

ESR2

Esr2; Esr2; Erb2; ERbeta; ER-beta; Erbeta; Nr3a2; ER-beta

ESR2_RAT

Rat

485

This assay has high sensitivity and excellent specificity for detection of Rat ESR2. No significant cross-reactivity or interference between Rat ESR2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 23.5 pg/ml-1500 pg/ml. Sensitivity: 5.8 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transcription/Transcription regulation. Protein Biological Process 3: Transcription

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ESR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ESR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ESR2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ESR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

6978817

NP_036886.1

NM_012754.1

Q62986

59,152 Da

Androgen Receptor Signaling Pathway (198529); Gene Expression Pathway (574364); Generic Transcription Pathway (574365); Nuclear Receptor Transcription Pathway (574367); Nuclear Receptors Pathway (198498); Ovarian Infertility Genes Pathway (198450)

binds estrogen and mediates transcriptional activation [RGD, Feb 2006]

ER-beta: a nuclear hormone receptor and transcription factor. Binds and activated by estrogen. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Binds estrogens with an affinity similar to that of ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Eight alternatively-spliced isoforms have been described. Isoform beta-cx lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability. Protein type: DNA-binding; Nuclear receptor. Cellular Component: neuron projection; mitochondrion; cell soma; perinuclear region of cytoplasm; cytoplasm; plasma membrane; perikaryon; nucleus; cilium. Molecular Function: peroxisome proliferator activated receptor binding; ligand-dependent nuclear receptor activity; estrogen receptor activity; hormone binding; zinc ion binding; estrogen response element binding; drug binding; steroid binding; protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor activity. Biological Process: prostate gland development; hypothalamus development; behavioral fear response; estrogen receptor signaling pathway; positive regulation of apoptosis; induction of apoptosis by hormones; negative regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; response to hormone stimulus; neuron migration; amygdala development; negative regulation of transcription from RNA polymerase II promoter; response to insecticide; uterus development; response to organic cyclic substance; response to estradiol stimulus; negative regulation of cell proliferation; learning and/or memory; ovarian follicle development; regulation of transcription, DNA-dependent; positive regulation of epidermal growth factor receptor signaling pathway; regulation of neuron apoptosis; cerebellum development; female gonad development; negative regulation of epithelial cell proliferation; response to nutrient levels; steroid hormone receptor signaling pathway; response to drug; transcription, DNA-dependent; male gonad development; vagina development; Sertoli cell proliferation; response to testosterone stimulus; Sertoli cell development; regulation of cell proliferation; response to genistein; response to ethanol; response to estrogen stimulus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; steroid hormone mediated signaling; brain development; response to activity; negative regulation of behavior; response to water deprivation

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800