catalog number :
MBS927957
products type :
ELISA Kit
products full name :
Bovine Microfibril-associated glycoprotein 4, MFAP4 ELISA Kit
products short name :
microfibrillar-associated protein 4
products name syn :
Bovine Microfibril-associated glycoprotein 4 (MFAP4) ELISA kit; microfibril-associated glycoprotein 4; microfibrillar-associated protein 4
other names :
microfibril-associated glycoprotein 4; Microfibril-associated glycoprotein 4; microfibril-associated glycoprotein 4; 36 kDa MAP; 36 kDa microfibril-associated glycoprotein; microfibrillar-associated protein 4; 36 kDa microfibril-associated glycoprotein; 36 kDa MAP
products gene name :
MFAP4
other gene names :
MFAP4; MFAP4; 36 kDa MAP
uniprot entry name :
MFAP4_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of bovine MFAP4. No significant cross-reactivity or interference between bovine MFAP4 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 62. 5 pg/ml -4000 pg/ml. Sensitivity: The minimum detectable dose of bovine MFAP4 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MFAP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MFAP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MFAP4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MFAP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001073686.1
ncbi gb acc num :
NM_001080217.1
ncbi mol weight :
28,558 Da
uniprot summary :
Function: Could be involved in calcium-dependent cell adhesion or intercellular interactions.
size4 :
10x96-Strip-Wells