Rat NADPH oxidase 1, NOX1 ELISA Kit | MyBioSource MBS927489 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat NADPH oxidase 1, NOX1 ELISA Kit

catalog :

MBS927489

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

product information

catalog number :

MBS927489

products type :

ELISA Kit

products full name :

Rat NADPH oxidase 1, NOX1 ELISA Kit

products short name :

[NADPH oxidase 1]

products name syn :

[Rat NADPH oxidase 1 (NOX1) ELISA kit; RP1-146H21.1; GP91-2; MOX1; NOH-1; NOH1; NADPH oxidase 1 variant NOH-1L; NADPH oxidase homolog-1; mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating) ; NADPH oxidase 1]

other names :

[NADPH oxidase 1; NADPH oxidase 1; NADPH oxidase 1; MOX1; MOX-1; NOH-1; NOX-1; mitogenic oxidase 1; NADH/NADPH mitogenic oxidase subunit p65-mox; NADPH oxidase 1; Mitogenic oxidase 1; MOX-1; NADH/NADPH mitogenic oxidase subunit P65-MOX; NOH-1]

products gene name :

[NOX1]

other gene names :

[Nox1; Nox1; Mox1; Noh1; NOX-1; MOX-1]

uniprot entry name :

NOX1_RAT

reactivity :

Rat

sequence length :

563

specificity :

This assay has high sensitivity and excellent specificity for detection of rat NOX1. No significant cross-reactivity or interference between rat NOX1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 125 pg/ml -8000 pg/ml. Sensitivity: 31.25 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NOX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NOX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NOX1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NOX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

16758500

ncbi acc num :

NP_446135.1

ncbi gb acc num :

NM_053683.1

uniprot acc num :

Q9WV87

ncbi mol weight :

65,177 Da

ncbi pathways :

Leukocyte Transendothelial Migration Pathway (83475); Leukocyte Transendothelial Migration Pathway (494); Osteoclast Differentiation Pathway (193140); Osteoclast Differentiation Pathway (193096); Phagosome Pathway (153901); Phagosome Pathway (153859)

ncbi summary :

involved in superoxide generation and serum-stimulated growth [RGD, Feb 2006]

uniprot summary :

NOX1: NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; EC 1.-.-.-; Oxidoreductase; Motility/polarity/chemotaxis; Channel, misc.; Membrane protein, integral. Cellular Component: early endosome; cytoplasm; cell junction; endosome; NADPH oxidase complex. Molecular Function: protein binding; metal ion binding; superoxide-generating NADPH oxidase activity; Rac GTPase binding; voltage-gated proton channel activity. Biological Process: intracellular pH elevation; cell migration; extracellular matrix organization and biogenesis; positive regulation of smooth muscle cell proliferation; superoxide metabolic process; positive regulation of JNK cascade; signal transduction; regulation of systemic arterial blood pressure by renin-angiotensin; response to reactive oxygen species; proton transport; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; hydrogen peroxide metabolic process; angiogenesis; positive regulation of integrin biosynthetic process; superoxide release

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145