Human 14-3-3 protein theta, YWHAQ ELISA Kit | MyBioSource MBS927389 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human 14-3-3 protein theta, YWHAQ ELISA Kit

catalog :

MBS927389

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS927389

products type :

ELISA Kit

products full name :

Human 14-3-3 protein theta, YWHAQ ELISA Kit

products short name :

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide

products name syn :

Human 14-3-3 protein theta (YWHAQ) ELISA kit; 14-3-3; 1C5; HS1; 14-3-3 protein T-cell; 14-3-3 protein tau; 14-3-3 protein theta; OTTHUMP00000165887; protein tau; tyrosine 3/tryptophan 5 -monooxygenase activation protein; theta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; theta polypeptide

other names :

14-3-3 protein theta; 14-3-3 protein theta; 14-3-3 protein theta; 14-3-3 protein tau; 14-3-3 protein T-cell; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1

products gene name :

YWHAQ

other gene names :

YWHAQ; YWHAQ; 1C5; HS1; 14-3-3

uniprot entry name :

1433T_HUMAN

reactivity :

Human

sequence length :

245

specificity :

This assay has high sensitivity and excellent specificity for detection of Human YWHAQ. No significant cross-reactivity or interference between Human YWHAQ and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates and cerebrospinal fluid (CSF). Assay Type: Sandwich. Detection Range: 0.47 ng/ml-30 ng/ml. Sensitivity: 0.11 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for YWHAQ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YWHAQ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAQ is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAQ bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

5803227

ncbi acc num :

NP_006817.1

ncbi gb acc num :

NM_006826.3

uniprot acc num :

P27348

ncbi mol weight :

27,764 Da

ncbi pathways :

Alpha6-Beta4 Integrin Signaling Pathway (198807); Calcium Regulation In The Cardiac Cell Pathway (198906); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Class I PI3K Signaling Events Mediated By Akt Pathway (138020); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); ErbB1 Downstream Signaling Pathway (138057); FoxO Family Signaling Pathway (138036); IL-3 Signaling Pathway (198881)

ncbi summary :

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]

uniprot summary :

14-3-3 theta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Associates with c-BCR and BCR-Abl. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 2p25.1. Cellular Component: focal adhesion; cytoplasmic vesicle membrane; membrane; cytoplasm; cytosol. Molecular Function: protein domain specific binding; protein binding; protein N-terminus binding. Biological Process: substantia nigra development; apoptosis; small GTPase mediated signal transduction; negative regulation of transcription, DNA-dependent; protein targeting

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage