ELISA/assay

Human Chymotrypsin-like elastase family member 1, ELA1 ELISA Kit

MBS927335

48-Strip-Wells

510 USD

ELISA Kit

Human Chymotrypsin-like elastase family member 1, ELA1 ELISA Kit

elastase 1, pancreatic

Human Chymotrypsin-like elastase family member 1 (ELA1) ELISA kit; ELA1; elastase 1; pancreatic; elastase 1; pancreatic

chymotrypsin-like elastase family member 1; Chymotrypsin-like elastase family member 1; chymotrypsin-like elastase family member 1; elastase-1; pancreatic elastase 1; elastase 1, pancreatic; chymotrypsin-like elastase family, member 1; Elastase-1; Pancreatic elastase 1

ELA1

CELA1; CELA1; ELA1; ELA1

CELA1_HUMAN

Human

258

This assay has high sensitivity and excellent specificity for detection of Human ELA1. No significant cross-reactivity or interference between Human ELA1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell culture supernates and Cell lysates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml-40 ng/ml. Sensitivity: 0.156 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ELA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ELA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ELA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ELA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

58331209

NP_001962.3

NM_001971.5

Q9UNI1

27,798 Da

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. To date, elastase 1 expression has only been detected in skin keratinocytes. Clinical literature that describes human elastase 1 activity in the pancreas or fecal material is actually referring to chymotrypsin-like elastase family, member 3B. [provided by RefSeq, May 2009]

CELA1: Acts upon elastin. Belongs to the peptidase S1 family. Elastase subfamily. Protein type: EC 3.4.21.36; Secreted, signal peptide; Protease; Secreted. Chromosomal Location of Human Ortholog: 12q13. Cellular Component: extracellular region. Molecular Function: metal ion binding; serine-type endopeptidase activity. Biological Process: Wnt receptor signaling pathway; multicellular organism growth; regulation of cell differentiation; exocrine pancreas development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; inflammatory response; proteolysis; regulation of cell proliferation; post-embryonic development

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800