catalog number :
MBS927070
products type :
ELISA Kit
products full name :
Rat Noggin, NOG ELISA Kit
products short name :
noggin
products name syn :
Rat Noggin (NOG) ELISA kit; SYM1; SYNS1; symphalangism 1 (proximal) ; noggin
other names :
Noggin; Noggin; noggin; noggin
other gene names :
Nog; Nog
uniprot entry name :
NOGG_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat NOG. No significant cross-reactivity or interference between rat NOG and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of rat NOG is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NOG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NOG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NOG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NOG bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
16,139 Da
ncbi pathways :
Signal Transduction Pathway (574116); Signaling By BMP Pathway (574211); TGF Beta Signaling Pathway (198444); TGF-beta Signaling Pathway (83456); TGF-beta Signaling Pathway (475)
ncbi summary :
secreted protein; involved in dorsal fate specification [RGD, Feb 2006]
uniprot summary :
Function: Essential for cartilage morphogenesis and joint formation. Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite
size4 :
10x96-Strip-Wells