Rat Receptor tyrosine-protein kinase erbB-2, ERBB2 ELISA Kit | MyBioSource MBS926985 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Receptor tyrosine-protein kinase erbB-2, ERBB2 ELISA Kit

catalog :

MBS926985

quantity :

96 Strip Wells

price :

760 USD

product information

catalog number :

MBS926985

products type :

ELISA Kit

products full name :

Rat Receptor tyrosine-protein kinase erbB-2, ERBB2 ELISA Kit

products short name :

v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

products name syn :

Rat Receptor tyrosine-protein kinase erbB-2 (ERBB2) ELISA kit; CD340; HER-2; HER-2/neu; HER2; NEU; NGL; TKR1; c-erb B2/neu protein; erbB-2; herstatin; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2; neuro/glioblastoma derived oncogene homolog (avian)

other names :

Receptor tyrosine-protein kinase erbB-2; Receptor tyrosine-protein kinase erbB-2; receptor tyrosine-protein kinase erbB-2; p185neu; C-erbB-2; p185erbB2; NEU proto-oncogene; proto-oncogene NEU; proto-oncogene c-ErbB-2; epidermal growth factor receptor-related protein; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; Avian erythroblastosis viral (v-erb-B2) oncogene homologue 2 (neuro/glioblastoma derived oncogene homolog); v-erb-b2 avian erythroblastic leukemia viral oncogene 2; Epidermal growth factor receptor-related protein; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; p185erbB2; p185neu; CD_antigen: CD340

products gene name :

ERBB2

other gene names :

Erbb2; Erbb2; Neu

uniprot entry name :

ERBB2_RAT

reactivity :

Rat

sequence length :

1257

specificity :

This assay has high sensitivity and excellent specificity for detection of Rat ERBB2. No significant cross-reactivity or interference between Rat ERBB2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm

other info2 :

Sample Volume: 50-100ul. Protein Biological Process 1: Transcription/Transcription regulation. Protein Biological Process 3: Transcription

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ERBB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ERBB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ERBB2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ERBB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

3915663

ncbi acc num :

P06494.3

uniprot acc num :

P06494

ncbi mol weight :

138,832 Da

ncbi pathways :

Adherens Junction Pathway 83462!!Adherens Junction Pathway 481!!Alpha6-Beta4 Integrin Signaling Pathway 198503!!Axon Guidance Pathway 574273!!Bladder Cancer Pathway 83503!!Bladder Cancer Pathway 527!!Calcium Signaling Pathway 83442!!Calcium Signaling Pathway 459!!Cardiovascular Signaling Pathway 219769!!Developmental Biology Pathway 574272

ncbi summary :

may play a role in cell proliferation and differentiation [RGD, Feb 2006]

uniprot summary :

Function: Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization 1 PublicationManual assertion based on experiment in:Ref.5. Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

size :

96 Strip Wells

price :

760 USD