Mouse Methylenetetrahydrofolate reductase, MTHFR ELISA Kit | MyBioSource MBS926900 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Methylenetetrahydrofolate reductase, MTHFR ELISA Kit

catalog :

MBS926900

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS926900

products type :

ELISA Kit

products full name :

Mouse Methylenetetrahydrofolate reductase, MTHFR ELISA Kit

products short name :

5, 10-methylenetetrahydrofolate reductase (NADPH)

products name syn :

Mouse Methylenetetrahydrofolate reductase (MTHFR) ELISA kit; 5; 10-methylenetetrahydrofolate reductase; OTTHUMP00000002367; 5; 10-methylenetetrahydrofolate reductase (NADPH)

other names :

methylenetetrahydrofolate reductase isoform b; Methylenetetrahydrofolate reductase; methylenetetrahydrofolate reductase; 5,10-methylenetetrahydrofolate reductase

products gene name :

MTHFR

other gene names :

Mthfr; Mthfr; AI323986

uniprot entry name :

MTHR_MOUSE

reactivity :

Mouse

sequence length :

654

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse MTHFR. No significant cross-reactivity or interference between mouse MTHFR and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of mouse MTHFR is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MTHFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MTHFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTHFR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTHFR bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

31543271

ncbi acc num :

NP_034970.2

ncbi gb acc num :

NM_010840.3

uniprot acc num :

Q9WU20

ncbi mol weight :

74,580 Da

ncbi pathways :

Folic Acid Network Pathway (198383); Metabolism Pathway (574739); Metabolism Of Folate And Pterines Pathway (574860); Metabolism Of Vitamins And Cofactors Pathway (574850); Metabolism Of Water-soluble Vitamins And Cofactors Pathway (574851); One Carbon Metabolism Pathway (198340); One Carbon Metabolism And Related Pathways (198380); One Carbon Pool By Folate Pathway (83206); One Carbon Pool By Folate Pathway (393); Folate Transformations I Pathway (142886)

uniprot summary :

MTHFR: Catalyzes the conversion of 5,10- methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co- substrate for homocysteine remethylation to methionine. Defects in MTHFR are the cause of methylenetetrahydrofolate reductase deficiency (MTHFRD). MTHFRD is autosomal recessive disorder with a wide range of features including homocysteinuria, homocysteinemia, developmental delay, severe mental retardation, perinatal death, psychiatric disturbances, and later-onset neurodegenerative disorders. Defects in MTHFR may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in MTHFR may be a cause of susceptibility to folate-sensitive neural tube defects (FS-NTD). The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Belongs to the methylenetetrahydrofolate reductase family. Protein type: Cofactor and Vitamin Metabolism - one carbon pool by folate; Energy Metabolism - methane; EC 1.5.1.20; Oxidoreductase. Cellular Component: neuron projection; cytosol. Molecular Function: FAD binding; oxidoreductase activity; NADP binding; methylenetetrahydrofolate reductase (NADPH) activity; catalytic activity. Biological Process: tetrahydrofolate metabolic process; metabolic process; methionine biosynthetic process; methionine metabolic process; homocysteine metabolic process; one-carbon compound metabolic process

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage