Mouse tyrosine hydroxylase, TH ELISA Kit | MyBioSource MBS926829 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse tyrosine hydroxylase, TH ELISA Kit

catalog :

MBS926829

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS926829

products type :

ELISA Kit

products full name :

Mouse tyrosine hydroxylase, TH ELISA Kit

products short name :

tyrosine hydroxylase (TH)

products name syn :

Mouse tyrosine hydroxylase (TH) ELISA Kit; DYT14; DYT5b; TYH; dystonia 14; tyrosine 3-monooxygenase; tyrosine hydroxylase (TH)

other names :

tyrosine 3-monooxygenase; Tyrosine 3-monooxygenase; tyrosine 3-monooxygenase; tyrosine 3-hydroxylase; tyrosine hydroxylase; Tyrosine 3-hydroxylase; TH

products gene name :

other gene names :

Th; Th; TH

uniprot entry name :

TY3H_MOUSE

reactivity :

Mouse

sequence length :

498

specificity :

This assay has high sensitivity and excellent specificity for detection of Mouse TH. No significant cross-reactivity or interference between Mouse TH and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates and cell culture supernates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml-40 ng/ml. Sensitivity: 0.156 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: biogenic amine biosynthesis and metabolism. Protein Biological Process 3: Catecholamine biosynthesis

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TH bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

6678337

ncbi acc num :

NP_033403.1

ncbi gb acc num :

NM_009377.1

uniprot acc num :

P24529

ncbi mol weight :

55,993 Da

ncbi pathways :

Alcoholism Pathway (585577); Alcoholism Pathway (587116); Amine-derived Hormones Pathway (574876); Amphetamine Addiction Pathway (547634); Amphetamine Addiction Pathway (550546); Biogenic Amine Synthesis Pathway (198386); Catecholamine Biosynthesis Pathway (574877); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (421757); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (468235); Cocaine Addiction Pathway (546267)

uniprot summary :

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described. Protein type: Amino Acid Metabolism - tyrosine; Endoplasmic reticulum; Vesicle; EC 1.14.16.2; Oxidoreductase; Mitochondrial. Cellular Component: internal side of plasma membrane; neuron projection; cytoplasmic vesicle membrane; smooth endoplasmic reticulum; mitochondrion; dendrite; melanosome membrane; terminal button; perikaryon; cytosol; synaptic vesicle; cell soma; axon; cytoplasm; cytoplasmic vesicle; nucleus. Molecular Function: protein domain specific binding; ferric iron binding; metal ion binding; ferrous iron binding; oxidoreductase activity; oxygen binding; tyrosine 3-monooxygenase activity; amino acid binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen; enzyme binding; iron ion binding; dopamine binding; monooxygenase activity. Biological Process: synaptic transmission, dopaminergic; eating behavior; heart development; mating behavior; locomotory behavior; learning; dopamine biosynthetic process; memory; synaptic vesicle amine transport; regulation of heart contraction; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; organ morphogenesis; eye photoreceptor cell development; response to ethanol; catecholamine biosynthetic process; visual perception; aromatic amino acid family metabolic process; response to hypoxia; embryonic camera-type eye morphogenesis; epinephrine biosynthetic process; neurotransmitter biosynthetic process

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage