Human Protein sel-1 homolog 1, SEL1L ELISA Kit | MyBioSource MBS926794 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Protein sel-1 homolog 1, SEL1L ELISA Kit

catalog :

MBS926794

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS926794

products type :

ELISA Kit

products full name :

Human Protein sel-1 homolog 1, SEL1L ELISA Kit

products short name :

sel-1 suppressor of lin-12-like (C. elegans)

products name syn :

Human Protein sel-1 homolog 1 (SEL1L) ELISA kit; IBD2; PRO1063; SEL1-LIKE; SEL1L1; Suppressor of lin 12 (sel-1); C. elegans; homolog of; sel-1 (suppressor of lin-12; C.elegans) -like; sel-1 suppressor of lin-12-like; sel-1 suppressor of lin-12-like 1; sel-1 suppressor of lin-12-like (C. elegans)

other names :

protein sel-1 homolog 1 isoform 2; Protein sel-1 homolog 1; protein sel-1 homolog 1; sel-1 suppressor of lin-12-like 1; suppressor of lin-12-like protein 1; Suppressor of lin 12 (sel-1), C. elegans, homolog of; sel-1 suppressor of lin-12-like (C. elegans); Suppressor of lin-12-like protein 1; Sel-1L

products gene name :

SEL1L

other gene names :

SEL1L; SEL1L; SEL1L1; PRO1063; SEL1-LIKE; TSA305; UNQ128/PRO1063; Sel-1L

uniprot entry name :

SE1L1_HUMAN

reactivity :

Human

sequence length :

301

specificity :

This assay has high sensitivity and excellent specificity for detection of human SEL1L. No significant cross-reactivity or interference between human SEL1L and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml -1200 pg/ml. Sensitivity: The minimum detectable dose of human SEL1L is typically less than 4.7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SEL1L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEL1L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEL1L is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEL1L bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

350529338

ncbi acc num :

NP_001231913.1

ncbi gb acc num :

NM_001244984.1

uniprot acc num :

Q9UBV2

ncbi mol weight :

88,755 Da

ncbi pathways :

HRD1/SEL1 ERAD Complex Pathway (413468); HRD1/SEL1 ERAD Complex Pathway (468401); Pre-NOTCH Expression And Processing Pathway (576265); Pre-NOTCH Processing In Golgi Pathway (576268); Protein Processing In Endoplasmic Reticulum Pathway (167325); Protein Processing In Endoplasmic Reticulum Pathway (167190); Signal Transduction Pathway (477114); Signaling By NOTCH Pathway (106485)

ncbi summary :

The protein encoded by this gene is part of a protein complex required for the retrotranslocation or dislocation of misfolded proteins from the endoplasmic reticulum lumen to the cytosol, where they are degraded by the proteasome in a ubiquitin-dependent manner. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

uniprot summary :

SEL1L: May play a role in Notch signaling. May be involved in the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Belongs to the sel-1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 14q31. Cellular Component: endoplasmic reticulum membrane; integral to membrane. Molecular Function: protein binding. Biological Process: Notch signaling pathway; ER-associated protein catabolic process

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage