catalog number :
MBS926712
products type :
ELISA Kit
products full name :
Human Apolipoprotein M, APOM ELISA Kit
products short name :
apolipoprotein M
products name syn :
Human Apolipoprotein M (APOM) ELISA kit; DADB-127H9.5; G3a; HSPC336; MGC22400; NG20; NG20-like protein; alternative name: G3a; NG20; apolipoprotein M
other names :
apolipoprotein M isoform 2; Apolipoprotein M; apolipoprotein M; protein G3a; NG20-like protein; alternative name: G3a, NG20; apolipoprotein M; Protein G3a
products gene name :
APOM
other gene names :
APOM; APOM; G3a; NG20; apo-M; HSPC336; G3A; NG20; HSPC336; Apo-M; ApoM
uniprot entry name :
APOM_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human APOM. No significant cross-reactivity or interference between Human APOM and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml-100 ng/ml. Sensitivity: 0.39 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 3: Lipid transport
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for APOM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APOM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APOM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APOM bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001243098.1
ncbi gb acc num :
NM_001256169.1
ncbi mol weight :
21,253 Da
ncbi summary :
The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Alternate splicing results in both coding and non-coding variants of this gene. [provided by RefSeq, Jan 2012]
uniprot summary :
APOM: Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid. Plasma protein. Expressed in liver and kidney. Belongs to the calycin superfamily. Lipocalin family. Highly divergent. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 6p21.33. Cellular Component: integral to plasma membrane. Molecular Function: antioxidant activity; lipid transporter activity; phospholipid binding. Biological Process: cholesterol homeostasis; reverse cholesterol transport; response to glucose stimulus; lipoprotein metabolic process; cholesterol efflux
size4 :
10x96-Strip-Wells