catalog number :
MBS926689
products type :
ELISA Kit
products full name :
Mouse Cerebellin-1, CBLN1 ELISA Kit
products short name :
cerebellin 1 precursor
products name syn :
Mouse Cerebellin-1 (CBLN1) ELISA kit; cerebellin 1; cerebellin 1 precursor
other names :
cerebellin-1; Cerebellin-1; cerebellin-1; precerebellin; brain protein D3; cerebellin 1 precursor protein; Brain protein D3; PrecerebellinCleaved into the following 2 chains:Cerebellin; CER; [des-Ser1]-cerebellin; des-Ser1-cerebellin
products gene name :
CBLN1
other gene names :
Cbln1; Cbln1; AI323299; CER; des-Ser1-cerebellin
uniprot entry name :
CBLN1_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse CBLN1. No significant cross-reactivity or interference between Mouse CBLN1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml-500 pg/ml. Sensitivity: 1.95 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CBLN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CBLN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CBLN1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CBLN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_062600.2
ncbi gb acc num :
NM_019626.3
ncbi mol weight :
21,113 Da
uniprot summary :
CBLN1: Cerebellin-1 is required for synapse integrity and synaptic plasticity. Required for the ER export and secretion of CBLN3. Protein type: Secreted; Secreted, signal peptide. Cellular Component: postsynaptic membrane; membrane; cell; extracellular region; plasma membrane; synapse; cell junction. Molecular Function: protein binding; protein homodimerization activity. Biological Process: heterophilic cell adhesion; positive regulation of synaptogenesis; protein secretion; cerebellar granule cell differentiation
size4 :
10x96-Strip-Wells