ELISA/assay

Mouse Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, ENPP1 ELISA Kit

MBS926662

48-Strip-Wells

510 USD

ELISA Kit

Mouse Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, ENPP1 ELISA Kit

ectonucleotide pyrophosphatase/phosphodiesterase 1

Mouse Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 (ENPP1) ELISA kit; M6S1; NPP1; NPPS; PC-1; PCA1; PDNP1; Ly-41 antigen; alkaline phosphodiesterase 1; membrane component; chromosome 6; surface marker 1; phosphodiesterase I/nucleotide pyrophosphatase 1; plasma-cell membra; ectonucleotide pyrophosphatase/phosphodiesterase 1

PREDICTED: ectonucleotide pyrophosphatase/phosphodiesterase family member 1 isoform X1; Ectonucleotide pyrophosphatase/phosphodiesterase family member 1; ectonucleotide pyrophosphatase/phosphodiesterase family member 1; E-NPP 1; tiptoe walking; lymphocyte antigen 41; plasma-cell membrane glycoprotein PC-1; phosphodiesterase I/nucleotide pyrophosphatase 1; 1 ectonucleotide pyrophosphatase/phosphodiesterase 1; ectonucleotide pyrophosphatase/phosphodiesterase 1; Lymphocyte antigen 41; Ly-41; Phosphodiesterase I/nucleotide pyrophosphatase 1; Plasma-cell membrane glycoprotein PC-1Including the following 2 domains:Alkaline phosphodiesterase I (EC:3.1.4.1); Nucleotide pyrophosphatase (EC:3.6.1.9); NPPase

ENPP1

Enpp1; Enpp1; Pca; ttw; twy; M6S1; NPP1; Npps; PC-1; Ly-41; Pca-1; Pdnp1; C76301; CD203c; E-NPP1; AI428932; 4833416E15Rik; Npps; Pc1; Pdnp1; E-NPP 1; Ly-41; NPPase

ENPP1_MOUSE

Mouse

906

This assay has high sensitivity and excellent specificity for detection of mouse ENPP1. No significant cross-reactivity or interference between mouse ENPP1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: The minimum detectable dose of mouse ENPP1 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ENPP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ENPP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ENPP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ENPP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

568965117

XP_006512660.1

XM_006512597.1

P06802

103,176 Da

Endochondral Ossification Pathway (198336); Insulin Signaling Pathway (198368); Metabolism Pathway (574739); Metabolism Of Vitamins And Cofactors Pathway (574850); Metabolism Of Water-soluble Vitamins And Cofactors Pathway (574851); Nicotinate And Nicotinamide Metabolism Pathway (83212); Nicotinate And Nicotinamide Metabolism Pathway (400); Pantothenate And CoA Biosynthesis Pathway (83213); Pantothenate And CoA Biosynthesis Pathway (401); Purine Metabolism Pathway (83144)

This gene encodes a member of the nucleoside pyrophosphatase/phosphodiesterase family of enzymes that catalyzes the hydrolysis of pyrophosphate and phosphodiester bonds in nucleotide triphosphates and oligonucleotides, respectively, to generate nucleoside 5'-monophosphates. The encoded protein is a type II transmembrane glycoprotein that negatively regulates bone mineralization. Mice harboring a nonsense mutation in this gene, termed tiptoe walking (ttw), exhibit ectopic ossification of the spinal ligaments. The encoded protein binds to the insulin receptor, inhibits downstream signaling events and induces insulin resistance and glucose tolerance. This gene is located adjacent to a paralog on chromosome 10. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

ENPP1: Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5 triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3 ,5 -cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. Homodimer; disulfide-linked. Interacts with INSR. Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. At low concentrations of ATP, a phosphorylated intermediate is formed which inhibits further hydrolysis. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. Protein type: Carbohydrate Metabolism - starch and sucrose; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 3.6.1.9; Motility/polarity/chemotaxis; Phosphatase (non-protein); Membrane protein, integral; Nucleotide Metabolism - purine; Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.4.1; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Phosphodiesterase. Cellular Component: extracellular space; cell surface; membrane; integral to plasma membrane; lysosomal membrane; integral to membrane; extracellular region; plasma membrane. Molecular Function: nucleotide diphosphatase activity; phosphodiesterase I activity; protein homodimerization activity; hydrolase activity; zinc ion binding; metal ion binding; calcium ion binding; nucleoside-triphosphate diphosphatase activity; insulin receptor binding; polysaccharide binding; nucleic acid binding; catalytic activity; ATP binding; scavenger receptor activity. Biological Process: generation of precursor metabolites and energy; sequestering of triacylglycerol; metabolic process; nucleoside triphosphate catabolic process; negative regulation of insulin receptor signaling pathway; negative regulation of fat cell differentiation; bone remodeling; phosphate metabolic process; 3 -phosphoadenosine 5 -phosphosulfate metabolic process; negative regulation of glucose import; cellular phosphate ion homeostasis; cellular response to insulin stimulus; biomineral formation; negative regulation of ossification; negative regulation of protein amino acid autophosphorylation; immune response; negative regulation of cell growth; inorganic diphosphate transport; negative regulation of glycogen biosynthetic process

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800