catalog number :
MBS926507
products type :
ELISA Kit
products full name :
Monkey Growth hormone receptor, GHR ELISA Kit
products short name :
growth hormone receptor
products name syn :
Monkey Growth hormone receptor (GHR) ELISA kit; GHBP; growth hormone binding protein; serum binding protein; somatotropin receptor; growth hormone receptor
other names :
growth hormone receptor; Growth hormone receptor; growth hormone receptor; GH receptor; somatotropin receptor; Somatotropin receptorCleaved into the following chain:Growth hormone-binding protein; GH-binding protein; GHBPAlternative name(s):Serum-binding protein
other gene names :
GHR; GHR; GH receptor; GH-binding protein; GHBP
uniprot entry name :
GHR_MACMU
specificity :
This assay has high sensitivity and excellent specificity for detection of monkey GHR. No significant cross-reactivity or interference between monkey GHR and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 4.68 ng/ml-300 ng/ml. Sensitivity: The minimum detectable dose of monkey GHR is typically less than 1.17 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GHR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GHR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GHR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GHR bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001036132.1
ncbi gb acc num :
NM_001042667.1
ncbi mol weight :
71,328 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (86721); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (86747); Jak-STAT Signaling Pathway (488); Neuroactive Ligand-receptor Interaction Pathway (86723); Neuroactive Ligand-receptor Interaction Pathway (462)
uniprot summary :
Function: Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to, and activates the JAK2/STAT5 pathway
size4 :
10x96-Strip-Wells