Human Citrate synthase, mitochondrial, CS ELISA Kit | MyBioSource MBS926487 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Citrate synthase, mitochondrial, CS ELISA Kit

catalog :

MBS926487

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS926487

products type :

ELISA Kit

products full name :

Human Citrate synthase, mitochondrial, CS ELISA Kit

products short name :

citrate synthase

products name syn :

Human Citrate synthase; mitochondrial (CS) ELISA kit; citrate synthase; mitochondrial; citrate synthase

other names :

citrate synthase, mitochondrial; Citrate synthase, mitochondrial; citrate synthase, mitochondrial; citrate (Si)-synthase; citrate synthase; Citrate (Si)-synthase

products gene name :

other gene names :

CS; CS

uniprot entry name :

CISY_HUMAN

reactivity :

Human

sequence length :

466

specificity :

This assay has high sensitivity and excellent specificity for detection of human CS. No significant cross-reactivity or interference between human CS and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml -500 pg/ml. Sensitivity: The minimum detectable dose of human CS is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CS bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

38327625

ncbi acc num :

NP_004068.2

ncbi gb acc num :

NM_004077.2

uniprot acc num :

O75390

ncbi mol weight :

51,712 Da

ncbi pathways :

Citrate Cycle (TCA Cycle) Pathway (82927); Citrate Cycle (TCA Cycle) Pathway (288); Citrate Cycle, First Carbon Oxidation, Oxaloacetate = 2-oxoglutarate Pathway (413347); Citrate Cycle, First Carbon Oxidation, Oxaloacetate = 2-oxoglutarate Pathway (468203); Citric Acid Cycle (TCA Cycle) Pathway (105919); Glyoxylate And Dicarboxylate Metabolism Pathway (83002); Glyoxylate And Dicarboxylate Metabolism Pathway (383); Metabolism Pathway (477135); Metabolism Of Proteins Pathway (106230); Mitochondrial Protein Import Pathway (576261)

ncbi summary :

The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq, Jul 2008]

uniprot summary :

CS: a mitochondrial enzyme of the tricarboxylic acid cycle that converts acetyl-CoA + H2O + oxaloacetate into citrate + CoA. Found in nearly all cells capable of oxidative metabolism. Protein type: Mitochondrial; EC 2.3.3.1; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Transferase; Carbohydrate Metabolism - citrate (TCA) cycle. Chromosomal Location of Human Ortholog: 12q13.2. Cellular Component: mitochondrion; mitochondrial matrix; nucleus. Molecular Function: citrate (Si)-synthase activity. Biological Process: cellular metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; cellular carbohydrate metabolic process

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage