Human Leukocyte surface antigen CD47, CD47 ELISA Kit | MyBioSource MBS926478 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Leukocyte surface antigen CD47, CD47 ELISA Kit

catalog :

MBS926478

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS926478

products type :

ELISA Kit

products full name :

Human Leukocyte surface antigen CD47, CD47 ELISA Kit

products short name :

CD47 molecule

products name syn :

Human Leukocyte surface antigen CD47 (CD47) ELISA kit; IAP; MER6; OA3; CD47 antigen; CD47 antigen (Rh-related antigen; integrin-associated signal transducer) ; CD47 glycoprotein; Rh-related antigen; antigen identified by monoclonal antibody 1D8; antigenic sur; CD47 molecule

other names :

leukocyte surface antigen CD47 isoform 1; Leukocyte surface antigen CD47; leukocyte surface antigen CD47; CD47 glycoprotein; Rh-related antigen; integrin associated protein; integrin-associated protein; integrin-associated signal transducer; antigenic surface determinant protein OA3; antigen identified by monoclonal antibody 1D8; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 molecule; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD_antigen: CD47

products gene name :

CD47

other gene names :

CD47; CD47; IAP; OA3; MER6; MER6; IAP

uniprot entry name :

CD47_HUMAN

reactivity :

Human

sequence length :

323

specificity :

This assay has high sensitivity and excellent specificity for detection of human CD47. No significant cross-reactivity or interference between human CD47 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, urine and tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 pg/ml -200 pg/ml. Sensitivity: 0.78 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD47 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD47 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD47 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD47 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4502673

ncbi acc num :

NP_001768.1

ncbi gb acc num :

NM_001777.3

uniprot acc num :

Q08722

ncbi mol weight :

35,214 Da

ncbi pathways :

Cell Surface Interactions At The Vascular Wall Pathway (106062); Cell-Cell Communication Pathway (477132); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Hemostasis Pathway (106028); Integrins In Angiogenesis Pathway (137999); Signal Regulatory Protein (SIRP) Family Interactions Pathway (187209)

ncbi summary :

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

uniprot summary :

CD47: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; Motility/polarity/chemotaxis; Membrane protein, integral. Chromosomal Location of Human Ortholog: 3q13.1-q13.2. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: protein binding. Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; response to bacterium; positive regulation of phagocytosis; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; opsonization; positive regulation of T cell activation; blood coagulation; cell adhesion; leukocyte migration; positive regulation of inflammatory response

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage