catalog number :
MBS926308
products type :
ELISA Kit
products full name :
Human Cytoskeleton-associated protein 4, CKAP4 ELISA Kit
products short name :
cytoskeleton-associated protein 4
products name syn :
Human Cytoskeleton-associated protein 4 (CKAP4) ELISA kit; CLIMP-63; ERGIC-63; MGC99554; p63; transmembrane protein (63kD); endoplasmic reticulum/Golgi intermediate compartment; type-II transmembrane protein p63; cytoskeleton-associated protein 4
other names :
cytoskeleton-associated protein 4; Cytoskeleton-associated protein 4; cytoskeleton-associated protein 4; 63 kDa membrane protein; type-II transmembrane protein p63; 63-kDa cytoskeleton-linking membrane protein; transmembrane protein (63kD), endoplasmic reticulum/Golgi intermediate compartment; cytoskeleton-associated protein 4; 63-kDa cytoskeleton-linking membrane protein; Climp-63; p63
products gene name :
CKAP4
other gene names :
CKAP4; CKAP4; p63; CLIMP-63; ERGIC-63; Climp-63; p63
uniprot entry name :
CKAP4_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human CKAP4. No significant cross-reactivity or interference between human CKAP4 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 23.44 pg/ml -1500 pg/ml. Sensitivity: 5.86 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CKAP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CKAP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CKAP4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CKAP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_006816.2
ncbi gb acc num :
NM_006825.3
ncbi mol weight :
66,022 Da
ncbi pathways :
Protein Processing In Endoplasmic Reticulum Pathway (167325); Protein Processing In Endoplasmic Reticulum Pathway (167190)
uniprot summary :
CKAP4: High-affinity epithelial cell surface receptor for APF. Protein type: Membrane protein, integral; Endoplasmic reticulum. Chromosomal Location of Human Ortholog: 12q23.3. Cellular Component: endoplasmic reticulum membrane; cytoskeleton; membrane; perinuclear region of cytoplasm; endoplasmic reticulum; integral to membrane; plasma membrane; lipid particle
size4 :
10x96-Strip-Wells