Mouse Integrin beta-1, ITGB1 ELISA Kit | MyBioSource MBS926185 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Integrin beta-1, ITGB1 ELISA Kit

catalog :

MBS926185

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS926185

products type :

ELISA Kit

products full name :

Mouse Integrin beta-1, ITGB1 ELISA Kit

products short name :

integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)

products name syn :

Mouse Integrin beta-1 (ITGB1) ELISA kit; CD29; FNRB; GPIIA; MDF2; MSK12; VLA-BETA; VLAB; fibronectin receptor beta subunit; integrin VLA-4 beta subunit; integrin beta 1; integrin; beta 1 (fibronectin receptor; beta polypeptide; antigen CD29 includes MDF2; MSK12)

other names :

integrin beta-1; Integrin beta-1; integrin beta-1; VLA-4 subunit beta; fibronectin receptor subunit beta; integrin beta 1 (fibronectin receptor beta); Fibronectin receptor subunit beta; VLA-4 subunit beta; CD_antigen: CD29

products gene name :

ITGB1

other gene names :

Itgb1; Itgb1; CD29; Fnrb; gpIIa; Gm9863; AA409975; AA960159; 4633401G24Rik; ENSMUSG00000051907

uniprot entry name :

ITB1_MOUSE

reactivity :

Mouse

sequence length :

798

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse ITGB1. No significant cross-reactivity or interference between mouse ITGB1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml -500 pg/ml. Sensitivity: The minimum detectable dose of mouse ITGB1 is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ITGB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ITGB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ITGB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ITGB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

45504394

ncbi acc num :

NP_034708.1

ncbi gb acc num :

NM_010578.2

uniprot acc num :

P09055

ncbi mol weight :

88,231 Da

ncbi pathways :

Adaptive Immune System Pathway (575096); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117303); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Axon Guidance Pathway (83262); Axon Guidance Pathway (476); Axon Guidance Pathway (575211); Bacterial Invasion Of Epithelial Cells Pathway (149817); Bacterial Invasion Of Epithelial Cells Pathway (148661); Basigin Interactions Pathway (575509); Cell Adhesion Molecules (CAMs) Pathway (83266)

uniprot summary :

ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle). Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral; Cell surface; Receptor, misc. Cellular Component: cell surface; integral to membrane; intercellular junction; lipid raft; hemidesmosome; membrane; perinuclear region of cytoplasm; cytoplasm; basement membrane; synapse; cytoplasmic vesicle; neuromuscular junction; integrin complex; receptor complex; endosome; filopodium; external side of plasma membrane; focal adhesion; acrosome; dendritic spine; cell projection; adherens junction; plasma membrane; cell junction; sarcolemma. Molecular Function: protein domain specific binding; protease binding; metal ion binding; laminin binding; receptor activity; alpha-actinin binding; actin binding; peptide binding; protein kinase binding; integrin binding; collagen binding; protein binding; protein heterodimerization activity; fibronectin binding; protein complex binding; cell adhesion molecule binding; glycoprotein binding; kinase binding; receptor binding. Biological Process: positive regulation of apoptosis; axon extension; regulation of cell cycle; multicellular organismal development; cell-matrix adhesion; cell fate specification; positive regulation of endocytosis; cardiac muscle cell differentiation; negative regulation of cell proliferation; leukocyte adhesion; positive regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; germ cell migration; tissue homeostasis; visual learning; cell adhesion; neurite development; negative regulation of cell projection organization and biogenesis; cardiac muscle development; integrin-mediated signaling pathway; cell migration; in utero embryonic development; dendrite morphogenesis; sarcomere organization; protein transport within lipid bilayer; cell-substrate adhesion; cellular calcium ion homeostasis; formation of radial glial scaffolds; heterotypic cell-cell adhesion; negative regulation of neuron differentiation; negative regulation of cell differentiation; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of Rho protein signal transduction; cell migration during sprouting angiogenesis; calcium-independent cell-matrix adhesion; stress fiber formation; positive regulation of neuron differentiation; leukocyte tethering or rolling; regulation of G-protein coupled receptor protein signaling pathway; G1/S transition of mitotic cell cycle; positive regulation of cell migration

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage