catalog number :
MBS926168
products type :
ELISA Kit
products full name :
Human Trefoil factor 3, TFF3 ELISA Kit
products short name :
trefoil factor 3 (intestinal)
products name syn :
Human Trefoil factor 3 (TFF3) ELISA kit; ITF; P1B; TFI; trefoil factor 3; trefoil factor 3 (intestinal)
other names :
trefoil factor 3; Trefoil factor 3; trefoil factor 3; intestinal trefoil factor; trefoil factor 3 (intestinal); Intestinal trefoil factor
products gene name :
TFF3
other gene names :
TFF3; TFF3; ITF
uniprot entry name :
TFF3_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human TFF3. No significant cross-reactivity or interference between Human TFF3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, urine, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml-100 ng/ml. Sensitivity: 0.39 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TFF3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TFF3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TFF3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TFF3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001107981.1
ncbi gb acc num :
NM_001114509.2
ncbi mol weight :
8,791 Da
uniprot summary :
Function: Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen)
size4 :
10x96-Strip-Wells