catalog number :
MBS926121
products type :
ELISA Kit
products full name :
Human CMRF35-like molecule 7, CD300LB ELISA Kit
products short name :
CD300 molecule-like family member b
products name syn :
Human CMRF35-like molecule 7 (CD300LB) ELISA kit; CD300b; CLM7; IREM3; TREM5; CD300 antigen like family member B; CD300 molecule like family member b; triggering receptor expressed on myeloid cells 5; CD300 molecule-like family member b
other names :
CMRF35-like molecule 7; CMRF35-like molecule 7; CMRF35-like molecule 7; leukocyte mono-Ig-like receptor 5; CD300 antigen like family member B; immune receptor expressed on myeloid cells 3; triggering receptor expressed on myeloid cells 5; CD300 molecule-like family member b; CD300 antigen-like family member B; CMRF35-A2; Immune receptor expressed on myeloid cells 3; IREM-3; Leukocyte mono-Ig-like receptor 5; Triggering receptor expressed on myeloid cells 5; TREM-5; CD_antigen: CD300b
products gene name :
CD300LB
other gene names :
CD300LB; CD300LB; CLM7; CLM-7; IREM3; TREM5; CD300b; IREM-3; TREM-5; CMRF35-A2; CD300B; CLM7; CMRF35A2; IREM3; LMIR5; TREM5; UNQ2530/PRO6029; CLM-7; IREM-3; TREM-5
uniprot entry name :
CLM7_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human CD300LB. No significant cross-reactivity or interference between human CD300LB and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 25 pg/ml-1600 pg/m. Sensitivity: The minimum detectable dose of human CD300LB is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD300LB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD300LB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD300LB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD300LB bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_777552.3
ncbi gb acc num :
NM_174892.3
ncbi mol weight :
22,689 Da
ncbi summary :
CD300LB is a nonclassical activating receptor of the immunoglobulin (Ig) superfamily expressed on myeloid cells (Martinez-Barriocanal and Sayos, 2006 [PubMed 16920917]).[supplied by OMIM, Mar 2008]
uniprot summary :
CD300LB: Acts as an activating immune receptor through its interaction with ITAM-bearing adapter TYROBP, and also independently by recruitment of GRB2. Belongs to the CD300 family. Protein type: Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 17q25.1. Cellular Component: plasma membrane; integral to membrane. Biological Process: innate immune response
size4 :
10x96-Strip-Wells